紫外线照射对小鼠创面TGFβ_1基因表达和蛋白含量影响的研究

Changes of ultraviolet-induced gene expression and content of transforming growth factor β_1 in burn wounds in mice

目的 研究紫外线照射不同次数对小鼠烫伤皮肤TGFβ1 基因表达及其蛋白含量的影响。方法 3 6只昆明种小鼠 (18～ 2 0g) ,随机分为 6组 :①正常皮肤组 (NS组 ) ;②烫伤后 1d对照组 (B1 C组 ) ;③烫伤后 7d对照组 (B7C组 ) ;④紫外线照射 1次组 (UV1 组 ) ;⑤紫外线照射 3次组 (UV3组 ) ;⑥紫外线照射 5次组 (UV5组 )。致B1 C、B7C、UV1 、UV3、UV5组小鼠皮肤深Ⅱ度烫伤 ,然后用紫外线以不同次数照射UV1 、UV3、UV5组小鼠烫伤创面 ,用原位杂交、免疫组化的方法检测TGFβ1 mRNA及其蛋白表达水平。结果 UV1 、UV3、UV5组TGFβ1 mRNA及蛋白含量明显增加。图象分析表明 :NS组、B1 C组均与UV1 、UV3、UV5组之间具有极显著性差异 (P <0 .0 0 1)。UV3、UV5组较UV1 组的TGFβ1 蛋白表达水平高 (P <0 .0 5 ) ,但UV3与UV5组之间无显著性差异 (P >0 .0 5 )。结论 紫外线可促进小鼠创面TGFβ1 表达 ,TGFβ1 的表达和蛋白含量的变化与创面愈合有关。

Objective To study the effect of different times of ultraviolet irradiation on the gene expression and content of transforming growth factor β 1(TGF β 1) in burn wounds in mice. MethodsThirty-six Kunming mice(18～20g) were randomly divided into 6 groups: A.normal skin group(NS);B.1st day after burn control group(B 1C);C.7th day after burn control group(B 7C);D.ultraviolet irradiation once group(UV 1);E.ultraviolet irradiation three times group(UV 3);F.ultraviolet irradiation five times group(UV 5).The deep Ⅱ degree burn wounds were artificially produced in the mice of groups B 1C, B 7C, UV 1 , UV 3 and UV 5.Then,different sessions of ultraviolet(432 mJ/cm2,D=50 cm,180 s) irradiation was applied on burn wounds in mice of groups UV 1, UV 3 and UV 5 at 24 hours after the burn. The level of TGF β 1 mRNA expression and protein were determined by situ hybridization and immunohistochemistry method. ResultsThe mRNA expression and protein of TGFβ 1 in groups UV 1, UV 3 and UV 5 were greatly increased. Image analysis showed that there were remarkable difference among the control group, the UV 1 group, UV 3 group and UV 5 group (P<0.001). In the UV 3 and UV 5 groups the content of TGF β 1was higher than that of the UV 1 group (P<0.05), but there was no difference between them (P>0.05). ConclusionThe findings showed that ultraviolet irradiation can promote the expression of TGFβ 1 in wound of mice. The gene expression and content of TGF β 1 were related with the healing of wounds.