先天畸形胎儿肺部病变的组织学观察和病因学探讨

Histological observation of the pulmonary lesions in congenital abnormal fetus and etiological study

目的 探讨先天畸形胎儿肺部病变特征及畸形与肺部感染的病因。方法 用苏木精 伊红染色观察肺部病变 ,用金葡菌及其L型免疫组化染色检测细菌及其抗原 ,用PCR技术检测肺炎组织中的金葡菌DNA。结果 2 1例先天畸形胎儿标本中 11例 (5 2 4 % )患有间质性肺炎 ,其中 4例 (19 0 % )PCR检测到金葡菌DNA ,5例 (2 3 8% )检测到金葡菌L型抗原 ,3例 (14 3% )免疫组化和PCR检测同时阳性。结论 金葡菌L型感染与胎儿畸形及其肺部感染可能有关。

Objective To explore the characteristics of the pulmonary lesions and the caurses of congenital malformation and pulmonary infection.Methods The pulmonary lesions stained with HE were observed and S.aureus and its L-form were detected with immunohistological and PCR techniques.Results Eleven cases of interstitial pneumonia were found in 21 cases of congenital malformation,among which S.aureus DNA were found in 4 cases,S.aureus L-form in 5 cases,both S.aureus DNA and S.aureus L-form in 3 cases.Conclusion S.aureus L-form infection might be related to the congenital malformation and its pulmonary infections.