三七总甙对家兔脑损伤组织NO、丙二醛含量的影响

EFFECTS OF PANAX NOTOG INSENG SAPONINS ON CONTENTS OF NO AND MDA FOLLOWING BRAIN INJURY IN RABBITS

目的:研究三七总皂甙(PNS)对减轻脑损伤后脑组织继发性损害的机理。方法:参照王荣丽方法检测家兔脑损伤后24h脑组织NO含量,改良八木国夫法检测脑组织丙二醛(MDA)含量,干湿法测定脑组织含水量,观察PNS对上述指标的影响。结果:脑损伤24h脑损伤组NO、MDA及含水量均高于脑损伤+PNS组和对照组,p<0.01。同时,脑组织中NO和MDA含量与含水量均呈显著正相关,p<0.01。脑损伤+PNS组和对照组脑组织中NO、MDA及含水量均无显著性差异。结论:PNS能通过降低脑损伤后NO及MDA的产生,减轻脑水肿的发生,对继发性脑组织损伤具有一定保护作用。

Objective: To study the effects of panax notoginseng saponins (PNS) on the secondary damage following brain injury. Method: A model of local cerebral injury was established by a drop 100 g weight at 20 cm height on the left forehead epidura in rabbits. Then the animals were divided into two groups, the injured group alone and injury + PNS group injected 100 mg/kg PNS intra- venously at 30 min and 12h after brain injury.The contents of nitric oxide (NO) and malondialcehyde (MDA) in the cerebral tissues of the animals were examined afte injury 24h, and compared with those of the normal animals. Results: The content of NO and MDA and wet brain tissue weight were higher in injury group than those in injury + PNS group and the normal group (p<0.01) .The content of NO and MDA were positively correlated to the water content in the brain tissues. While there were not any significant differences of NO, MDA and wet brain weight between injury +PNS group and the normal group. Conclusion; PNS can alleviate the brain edema through decreasing level of NO and MDA after brain injury, which may play a role in preventing secondary brain damage.