参麦活血饮对糖尿病大鼠坐骨神经和红细胞山梨醇水平及神经功能的影响

Effects of Shen Mai Huo Xue Yin on the sorbitol concentration of erythrocyte and sciatic nerve and on the nerve function in diabetic rats

目的 探讨糖尿病 (DM )大鼠坐骨神经和红细胞山梨醇含量与神经功能之间的关系 ,观察参麦活血饮进行短期干预的作用和可能机制。方法 采用链脲佐菌素诱发DM大鼠模型 ,造模1周后用中成药参麦活血饮 (成分为红参、麦冬、红花 )治疗。用药 4周后测定神经电生理、坐骨神经和红细胞山梨醇水平。结果 DM大鼠神经传导速度减慢 ,坐骨神经和红细胞山梨醇水平升高 ;经参麦活血饮治疗后上述改变减轻。同时 ,红细胞和坐骨神经山梨醇水平呈正相关 ;感觉神经传导速度与坐骨神经和红细胞山梨醇水平呈负相关 ,H反射与坐骨神经和红细胞山梨醇水平呈正相关 ;运动神经传导速度与坐骨神经和红细胞山梨醇水平无明显相关。结论 DM时红细胞山梨醇水平可反映坐骨神经山梨醇水平 ,亦能反映周围神经的病变程度。

Objective To investigate the relationship between the nerve function and the sorbitol concentrations of erythrocyte and sciatic nerve, and to evaluate the effects and mechanism of a formulae of Chinese medicine, Shen Mai Huo Xue Yin (a decoction, it ingredients including ginseng, tuber of dwarf lilyturf and safflower) on diabetic peripheral neuropathy (DPN). Methods The rats were divided into 3 groups: normal rats, diabetic rats and diabetic rats treated with Shen Mai Huo Xue Yin. The diabetes of rats was induced by streptozotocin intraperitoneally. Motor nerve conduction velocity (MNCV) of sciatic, sensory nerve conduction velocity (SNCV) of caudal nerve and the H reflex were detected with evoked electromyogram; The sorbitol concentrations of erythrocyte and sciatic nerve was measured by spectrophotography. Results The sorbitol concentration of erythrocyte was positively correlated with that in the sciatic nerve, and the sorbitol concentration of erythocyte and sciatic nerve were negatively correlated with SNCV and positively with H reflex. The SNCV reduced significantly and the sorbitol concentration of both erythrocyte and sciatic nerve increased significantly in diabetic group as compared with those in normal group. The SNCV and sorbitol concentrations of sorbitol both erythrocyte and sciatic nerve in diabetic group were significantly improved after treated with Shen Mai Huo Xue Yin. Conclusion The sorbitol concentration in erythrocyte reflects the level of that in sciatic nerve and the lesion degrees of DPN. Shen Mai Huo Xue Yin alleviates the lesion of DPN in the early stage.