雌激素对C6神经胶质瘤细胞的作用

Effects of estradiol on C6 glioma cell line

目的 从细胞与分子水平观察雌激素受体 (ER)α,β2种亚型在C6神经胶质瘤细胞的表达 ,初步研究雌激素对大鼠C6星形神经胶质瘤细胞的细胞活性、增殖活动和细胞[Ca2 + ]i 水平的影响 .方法 应用细胞培养、免疫细胞化学、RT PCR技术、MTT法、3 H TdR掺入法和激光扫描共聚焦技术 .结果 ①C6细胞中检测到ERβ免疫反应阳性物质 ,未发现ERα免疫反应阳性物质 ;②ERβmRNA在C6细胞中有表达 ,未检测到ERαmRNA ;③ 17β estradiol (10 -7～ 10 -5mol·L-1)可显著提高C6细胞活力 ,但对细胞增殖程度无明显的影响 ;④ 17β estradiol (10 -6mol·L-1)作用后数 10s后C6细胞 [Ca2 + ]i荧光强度显著增强 .结论 C6细胞表达ERβ的mRNA与蛋白 ,在神经胶质瘤细胞中ERβ可能是介导雌激素信号传递的关键环节 ;雌激素可发挥细胞保护作用 ;雌激素使细胞 [Ca2 + ]i增加 .

AIM To study the expression of protein and mRNA of estrogen receptor (ER) in C6 glioma cell line, and the effects of estradiol on the C6 cells. METHODS The techniques of cell culture, immunocytochemistry, RT PCR, MTT, 3H TdR incorporation and interactive laser cytometry were used. RESULTS ① Only ERβ immunoreactive production and no ER was detected in C6 cells. ② C6 cells expressed ERβmRNA and no ERαmRNA was found. ③ 17β estradiol (10 -7 ～10 -5 mol·L -1 ) promoted the viability of the cells with no effect on the cell proliferation. ④ 17β estradiol increased the intracellular fluorescence intensity of [Ca 2+ ] i in C6 cells. CONCLUSION C6 cells can express ERβ protein and mRNA. ERβ may play a key role in the signal transduction of estradiol in glioma cells. Estradiol has some protective effects on cells and can increase the [Ca 2+ ] i of C6 cells. All this findings provide more evidence for the role of estradiol in the central nervous system.