摘要
目的 观察HCVC区基因在SP2 / 0细胞及荷瘤小鼠瘤内的表达情况 .方法 构建pEF HCVC重组质粒 ,转染SP2 / 0细胞 ,经G4 18进行筛选 ,建立稳定转染pEF HCVC的SP2 / 0细胞系 .并将转染后的SP2 / 0细胞接种于BALB/c小鼠皮下产生浆细胞瘤 .结果 用斑点杂交方法检测转染后的细胞 ,有HCVmRNA .免疫组化检测瞬时转染以及稳定转染的SP2 / 0细胞涂片均发现存在有阳性信号 .另外 ,对瘤组织进行免疫组化检测 ,发现有HCVC蛋白表达 .结论 pEF HCVC重组质粒可以在SP2 / 0细胞中长期稳定表达 ,也可以在BALB/c小鼠浆细胞瘤内表达 .
AIM To observe the expression of HCV core protein in SP2/0 cell and BALB/c mouse plasmacytoma. METHODS The recombinant plasmid pEF HCV was constructed and transfected into SP2/0 cells. After SP2/0 cells were screened by G418, a cell model with stable expression was established. The transfected SP2/0 cells were inoculated subcutaneously into the right and left flank of the syngeneic BALB/c mice to develop plamacytoma tumors. RESULTS Positive signals were detected by blot hybridization in SP2/0 transfected with pEF HCV. The cell besmears of SP2/0 cells transfected with pEF HCV core instantaneously and stably were detected by immunohistochemical staining. Positive signals were found in all the samples. Such was the case in SP2/0 plasmacytoma tumors. CONCLUSION Recombinant pEF HCV core can express stably in SP2/0 cells and BALB/c mouse plasmacytoma, and this model can be used as an animal model to evaluate the effects of anti HCV agents.
出处
《第四军医大学学报》
北大核心
2002年第24期2236-2239,共4页
Journal of the Fourth Military Medical University
基金
国家自然科学基金资助项目 (3980 0 1 2 1 )
