苏铁共生蓝细菌PCR条件的优化

Optimization of PCR Conditions for Symbiosis of Cyanobacteria in Cycads

在研究苏铁共生蓝细菌的种群多态性过程中 ,为了直接以极少量的新鲜蓝细菌为PCR反应模板 ,对 5种抗抑制剂进行了一系列优化组合方案的试验 ,并对比了 3种循环参数 ,发现在 2 5 μL标准反应液基础上加有 3.75 μL 10 %BSA和 2 μL 10 %DMSO ,退火温度为 5 6℃时效果最佳。

To optimize PCR conditions for symbiosis of cyanobateria isolated freshly from the coralloid roots of cycads, a series of comparative experiments were carried out. The results showed that the profiles of PCR products could be improved obviously by adding 3.75 μL 10% BSA and 2 μL 10% DMSO into the reaction systems with a melting temperature of 56℃.