摘要
目的 了解重组人肝再生增强因子 (hALR)对大鼠肝星状细胞间质胶原酶 (MMP1 3)基因表达的影响。方法 在培养的肝星状细胞系中加入 2 0 0、2 0、2 μg L 3种浓度的hALR ,于 8、2 4、48、72h 4个时间点收集细胞 ,提取总RNA ;用逆转录定量聚合酶链反应 (PCR )方法测定MMP1 3 的基因表达水平。结果 高、中、低浓度的hALR 3组肝星状细胞MMP1 3 基因表达水平在8、2 4、48、72h 4个时间点均明显高于对照组 ;最高值均出现在 2 4~ 48h ,低剂量组为对照组的 3倍 ,中剂量组为对照组的 4倍 ,高剂量组达对照组的 6倍。结论 重组人肝再生增强因子对大鼠肝星状细胞间质胶原酶基因表达有明显的促进作用。
Objective To investigate the effects of recombinant human augmentor of liver regeneration (hALR) on interstitial collagenase(MMP 13) gene expression in hepatic stellate cells.Methods Hepatic stellate cells (HSC-T6) were cultured in medium containing different concentrations of hALR (200,20,2 μg/L respectively) and cells were collected at different incubation periods (8,24,48,72 h).Total RNA of HSC was isolated and MMP 13 gene expressions level was detected by reverse transcription polymerase chain reaction.Results MMP 13 gene expression levels of HSC in high (200 μg/L),mid (20 μg/L) and low (2 μg/L) concentrations of three hALR groups were much higher than those in the control group after exposure to hALR at 8,24,48 and 72 h.The maximum values appeared at 24 to 48 h.The expression level in low dose group,mid dose group and high dose group was 3 times,4 times and 6 times of control group,respectively.Conclusion hALR could promote gene expression of interstitial collagenase in hepatic stellate cells.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2003年第1期14-15,共2页
Chinese Journal of Experimental Surgery
基金
军队"十五"杰出人才基金课题 (0 1J0 0 2 )