MAGE-3抗原肽体外诱导肝癌患者免疫应答的研究

Peptide derived from MAGE-3 epitope induced cytotoxic T lymphocytes of hepatocellular carcinoma patient in vitro

的 利用载有MAGE 3抗原肽的树突状细胞 (DC)活化原发性肝细胞癌 (HCC)患者T淋巴细胞 ,探讨是否可以体外诱导特异性细胞毒T淋巴细胞 (CTL)应答。方法 通过逆转录多聚酶链反应和测序分析MAGE 3多肽表位密集区的核苷酸变异状况 ;体外培养HLA A2表型的HCC患者及正常献血员外周血来源的DC ,并经孵育携带MAGE 3 HLA A2抗原肽FLWGPRALV ,用以活化T淋巴细胞 ,利用特异性杀伤实验检测CTL应答。结果 中国HCC患者表达的MAGE 3序列高度保守。用特定细胞因子和无血清培养基可成功培养HCC患者外周血来源的DC。经多肽冲击的DC诱导 ,3例HCC患者和 3例正常献血员中各有 2例产生CTL免疫应答。结论 载有MAGE 3抗原肽的DC可以体外诱导特异性的CTL免疫应答。提示HLA A2限制性的MAGE 3抗原肽经DC递呈可以作为有潜力的肝癌免疫治疗疫苗。

Objective To investigate whether the cytotoxic T lymphocytes (CTLs) response can be induced by MAGE-3-loaded dendritic cells in vitro activating the T lymphocytes in hepatocellular carcinoma patients.Methods The encoding nucleotides of MAGE-3 epitopes were analyzed by RT-PCR assay,cloning and sequencing.Dendritic cells (DCs) were induced from peripheral blood mononuclear cells of 3 HCC patients and 3 healthy donors with HLA-A2 phenotypes,and cultured with GM-SCF and IL-4 in AIM-V medium.The expanded DCs were pulsed by MAGE3/HLA-A2 peptide (FLWGPRALV) and exposed to the radiation with a dose of 3?000 rads.Irradiated DCs were used to stimulate autologous T lymphocytes for three times.Then the induced CTLs were evaluated by killing peptide-pulsed T2 cells.Results Compared with the sequences in Genbank,the MAGE-3 coding genes isolated from Chinese patients were highly conserved.The MAGE-3 peptide-loaded DCs can induce specific CTLs successfully in 2 of 3 HCC patients and 2 of 3 healthy donors.Conclusion MAGE-3 peptide-loaded DCs can activate T lymphocytes and induce peptide-specific CTL response,and is a potential target for specific immunotherapy for HCC.