蛋白激酶C活性及δ亚型在肾透明细胞癌的表达

Clincal significance of protein kinase C and protein kinase C-δexpression in renal clear cell carcinoma

目的 探讨蛋白激酶C(PKC)、PKC δ在肾透明细胞癌中的表达及其临床意义。方法 采用PKC比活性测定和WesternBlotting方法检测 38例肾透明细胞癌和 5例正常肾组织中PKC比活性和PKC δ的表达。结果 癌组织和正常组织胞浆PKC比活性值分别为 ( 6 .97± 2 .6 5 )10 - 2 pmol·ml- 1 ·min- 1 和 ( 14 .16± 5 .6 7) 10 - 2 pmol·ml- 1 ·min- 1 ;而胞膜中的比活性值分别为( 2 7.90± 7.5 8) 10 - 2 pmol·ml- 1 ·min- 1 和 ( 5 7.0 6± 18.13) 10 - 2 pmol·ml- 1 ·min- 1 ;PKC δ在癌组织细胞胞浆中的相对灰度值小于 1,在胞膜中则大于 1,各病理分期和细胞分级间差异均有显著性 (P<0 .0 5 )。结论 PKC活性在肾透明细胞癌中下降 ,PKC δ表达与病理分期、细胞分级相关 ,是判断肾细胞癌预后的指标。

Objective To study the clinical significance of protein kinase C (PKC) specific activity and expression of PKC-δin renal clear cell carcinoma (RCCC).Methods Specific activity of PKC was detected by isotope emitting and expression of PKC-δby Western Blotting in 38 cases of RCCC and 5 cases of normal tissues.Results specific activities of PKC in cytoplasm of RCCC and normal tissue were ( 6.97±2.65)10 -2 pmol/(ml.min) and (14.16±5.67)10 -2 pmol/(ml.min) respectively;while those in membrane were (27.90±7.58)10 -2pmol/(ml·min) and (57.06±18.13)10 -2 pmol/(ml.min) respectively;the ratio of PKC-δbetween RCCC and normal tissue was less than 1 in cytoplasm,and more than 1 in membrane.There was signficant differenc in PKC-δ among different stages and grades(P<0.05).Conclusion Specific activity of PKC was decreased in RCCC,and PKC-δwas related to clinical stages and pathological grades as an effective index for tumor prognosis.