P物质对大鼠成纤维细胞碱性成纤维细胞生长因子及其受体表达的影响

The effect of substance P on basic fibroblast growth factor and typeⅠreceptor gene expressions in rat fibroblasts in vitro

目的 探讨P物质 (SP)对大鼠肉芽组织成纤维细胞碱性成纤维细胞生长因子 (bF GF)及其受体 (FGFR 1)表达的影响。方法 采用成纤维细胞体外培养和逆转录 多聚酶链反应(RT PCR)技术 ,观察SP在不同浓度 ( 1× 10 - 9～ 1× 10 - 5mol L)及孵育时间 ( 0、3、6、12、2 4h)情况下刺激成纤维细胞后 ,其bFGF、FGFR 1mRNA表达情况。结果 SP可上调大鼠成纤维细胞bF GF、FGFR 1mRNA表达。SP对bFGF的量 效曲线呈双相分布 ,最大效应浓度为 1× 10 - 7mol L。但SP仅在高浓度 ( 1× 10 - 6 ～ 1× 10 - 5mol L)时促进FGFR 1表达。在最大效应浓度 ( 1× 10 - 7～ 1× 10 - 5mol L)时 ,SP对bFGF、FGFR 1表达的上调作用分别于刺激细胞后 3、12h达高峰。结论 SP对大鼠肉芽组织成纤维细胞bFGF、FGFR 1基因表达存在直接影响 ,其表现形式与SP的刺激浓度及孵育时间有关 ,这可能是SP在创伤修复中发挥作用的机制之一。

Objective To probe into the effect of substance P(SP) on basic fibroblast growth factor(bFGF) and fibroblast growth factor receptor-1(FGFR-1) gene expression in rat granulation tissue fibroblasts.Methods RT-PCR was used to observe changes of bFGF and FGFR-1 gene expressions in fibroblasts treated with SP under different stimulating concentrations(1×10 -9to 1×10 -5mol/L)and incubating time points(0,3,6,12,24 h).Results SP could up regulate bFGF and FGFR-1 gene expressions in fibroblasts in vitro.The response curve that SP affected bFGF,displayed a two way distribution with a most effective dot in 1×10 -7mol/L,but SP only up regulated FGFR-1 gene expression at higher concentrations(1×10 -6to 1×10 -5 mol/L).After stimulating fibroblast with 1×10 -7to 1×10 -5mol/L SP,bFGF and FGFR-1 gene expressions achieved their peak time points at 3,12 h respectively.Conclusion SP had a direct effect on bFGF and FGFR-1 gene expression in rat granulation tissue fibroblasts in vitro,relative to SP stimulating concentrations and incubating time.This effect may be one of mechanisms of SP biological functions during wound healing.