成人急性白血病患者骨髓中p16基因的表达

Expression of p16 gene in adult acute leukemia

目的:研究成人急性白血病患者p16基因纯合缺失和甲基化变化与其表达之间的关系,探讨其在成人急性白血病发生发展中的生物学意义。方法:分别用多重聚合酶联反应(PCR)技术及甲基化敏感限制性内切酶-PCR技术检测了71例成人急性白血病患者DNA中p16基因纯合缺失及甲基化情况;应用原位杂交及免疫化学技术检测p16mRNA及p16蛋白表达。结果:71例成人急性白血病患者中,2例检测出p16基因纯合缺失。在无p16基因纯合缺失的病例中,5例急性淋巴细胞白血病(5/26)和9例急性髓性白血病(9/43)患者测出p16基因甲基化。p16 mRNA及P16蛋白的阳性率分别为70.4％(50/71例)、61.9％(44/71例)。结论:在成人急性白血病的发生发展中,p16基因甲基化比p16基因纯合缺失更具有意义;p16基因表达异常与成人急性白血病的发生发展密切相关。

Aim:To investigate the abnormality of p16 gene and its products in adult acute leukemia (AL) and to define its value. Methods:Bone marrow DNA obtained from 71 adults with AL(28 ALL and 43 AML) were studied with mul tiple comparative polymerase chain reaction and a restriction enzymes-PCR to detect the homozygous deletion and methyla-tion of p16 gene respectively. Hybridization in situ method and immunochemistry method were used to detect p16 mRNA and P16 protein respectively. Results: Homozygous loss of p16 gene was detected in 2 cases of B-ALL, and methylation was detected in 14 cases out of the other 69 cases without homozygous deletion of p16, which were 5 of 26 ALL and 9 of 43 AML. The ratio of positive expression of p16 mRNA and P16 protein were 70.4% (50/71 ) and 61. 9% (44/71 ) respectively. Conclusion:Methylation of p16 gene may play a more important role than homozygous deletion of p16 in the leukemogene-sis and progression of adult acute leukemia.