重组人白细胞介素-Ⅱ对大鼠长期毒性观察

Observation of long-term toxicity of recombinant human interleukin-Ⅱ in rats

目的:观察经腹腔注射重组人白细胞介素-Ⅱ(RHIL-2)90 d对大鼠的毒性。方法:60只大鼠随机均分成3组:对照组、低剂量组和高剂量组。低、高剂量分别腹腔注射RHIL-2 40万IU/(kg·d)和60万IU/(kg·d),对照组给予生理盐水,3组均连续用药90 d。分别观察动物的一般情况、血常规和病理组织学变化。结果:在用药全过程中,动物一般情况良好,RHIL-2对体重、白细胞计数、红细胞计数、血小板计数、血红蛋白含量、肝、肾功能及各主要脏器病理组织学均无明显影响。结论:结果提示长期应用RHIL-2不会产生蓄积毒性。

Aim : To observe the long-term toxicity of recombinant human interleukin- Ⅱ ( RHIL-2) after 90 days of in-traperitoneal (ip) administration in rats. Methods: Sixty rats were allocated into 3 groups: control group, low dose group, and high dose group. The dosage of the low dose group and high dose group were 40 and 60 ten thousand unit/( kg · d) ,respectively, and control group was given normal saline. The toxic sign, hemotologic, blood biochemical indexes, and histo-pathology were observed or determined at the 45th and 90th day of administration. Results: The results showed that during the whole experimental period, the general condition of rats was good. No noticeable toxicity was found in the number of leucocyte, red cell and platelet, homoglobin, liver kidney function test, or histopathological examination. Conclusion: RhIL-2 has no accumulated toxic effects in rats after a long-term administration.