丹参及丹参注射液指纹图谱的HPLC-MS研究

Study on fingerprints of Radix Salviae Miltiorrhiza and its INJECTION by HPLC-MS

目的 采用 HPL C- U V- MS法对丹参药材、丹参注射液中间体及丹参注射液进行指纹图谱的研究。方法 采用 Alltim a C1 8分析柱 ,甲醇 -水 -冰醋酸梯度洗脱系统 ,流速 :1m L / m in,检测波长 :2 81nm ,MS同时记录总离子流(TIC)色谱图。结果 得到分离度较好的丹参药材、中间体及注射液的 HPL C- UV及 HPL C- MS指纹图谱。结论 为丹参药材、中间体及注射液的质量控制提供全面、可靠的依据。

Object To investigate the chromatographic fingerprints of Radix Salviae Miltiorrhizae (RSM), its intermediate and INJECTION by HPLC-UV-MS. Methods Separation was performed on an Alltima C 18 analytical column with the mobile phase consisting of methanol-water-acetic acid as gradient eluent at the flow rate of 1 mL/min. The UV detection was set at 281 nm and TIC was recorded by an electrospray ionization mass spectrometer in negative ion mode. Results The HPLC-UV and HPLC-MS fingerprints of RSM, its intermediate and INJECTION were obtained with perfect isolation. Conclusion The fingerprints could be used for the control of RSM, its intermediate and INJECTION.