微量快速斑点免疫金银染色法检测系统性红斑狼疮患者血清肾小球基底膜抗体的研究

Detection of antibodies of glomerular basement membrane by MFDot-IGSS in patients with SLE

目的 建立一种便捷的检测系统性红斑狼疮 (SLE)患者血清肾小球基底膜抗体 (GBA Ab)的方法。 方法 在斑点免疫金银染色 (dot immunogoldsilverstaining ,Dot IGSS)的基础上 ,待检血清标本原倍使用 ,缩短抗原与抗体及与金标二抗的作用时间 ,建立微量快速斑点免疫金银染色法 (microvolume fast dot IGSS ,MFDot ISGG )。 结果 用MFDot IGSS法和Dot IGSS法同时检测SLE患者血清 6 0份 ,GBM Ab阳性率分别为 6 0 %和 6 1.7% ;检测其他自身免疫性疾病患者血清 40份 ,GBM Ab阳性率分别为 2 .5 %和 2 .5 % ;检测健康献血员血清 40份 ,GBM Ab均为阴性。二种方法的敏感性和特异性差异均无显著意义 (P >0 .0 5 )。 结论 MFDot IGSS法检测SLE患者血清GBM Ab的敏感性和特异性与Dot IGSS法相似 ,但操作简便快速 。

Objective To set up a simple and convenient immunoassay for the diagnosis of SLE.Methods A microvolume fast dot-immunogold silver staining(MFDot-IGSS) technique was set up on the basis of the conventional Dot-IGSS by omitting the incubation step to shorten the total reaction time by several hours and by using the sample sera undiluted to elevate the concentration.Results The positive rates of the GBM-Ab in 60 cases of patient with SLE were 60% by MFDot-IGSS and 61.7% by Dot-IGSS.The positive rates of the GBM-Ab in 40 cases of other autoimmune diseases(AID) were both 2.5%.The GBM-Ab in 40 healthy controls were negative.No significant differences were found in the results between the fast and the conventional assays(P<0.05).Conclusions The sensitivity and specificity of MFDot-IGSS were similar to those of the conventional Dot-IGSS while the former is simple,time-saving and suitable for clinics.