痹肿消汤对实验性关节炎大鼠滑膜VEGF表达水平的影响

Effect of bizhongxiao decoction on the expression of VEGF in the synovial membrane of CⅡ-induced rheumatoid arthritis in rats

目的 :观察痹肿消汤对实验性关节炎大鼠滑膜组织血管内皮生长因子 (VEGF)表达水平的影响 ,探讨痹肿消汤治疗类风湿性关节炎 (RA)的作用机制。方法 :75只SD大鼠随机分为 4组 ,皮下注射Ⅱ胶原诱导实验性关节炎模型 ,用免疫组化法检测各组不同时间点大鼠滑膜组织VEGF的表达 ,同时采用关节炎指数积分法评价其关节炎症状。结果 :免疫大鼠 88.5 7%出现关节炎症状 ;模型组的关节炎指数积分逐渐增加 ,而痹肿消汤组和甲氨喋呤组关节炎指数积分则在 30d后逐渐下降。正常组滑膜组织未见明显VEGF表达 ,模型组VEGF表达明显高于痹肿消汤组和甲氨喋呤组 (P <0 .0 1) ;随着时间的延长 ,模型组VEGF表达逐渐升高 ,痹肿消汤组和甲氨喋呤组的VEGF表达明显低于模型组 (P <0 .0 1) ,且痹肿消汤组又低于甲氨喋呤组 (P <0 .0 5 )。结论 :VEGF参与了RA疾病滑膜炎的形成和发展过程 ;其VEGF表达的高低与关节炎症状的轻重有关。痹肿消汤和甲氨喋呤均具有阻断关节炎滑膜组织VEGF表达 ,且痹肿消汤阻断作用优于甲氨喋呤 ;

Objective To observe the effect of bizhongxiao (BZX) decoction on the expression of VEGF in the synovial membrane of CⅡ induced arthritis(CIA) in rats and to explore the mechanism of BZX decoction in the treatment of rheumatoid arthritis RA. Methods Seventy five SD rats were randomly divided into four groups.The rat experimental arthritis model was established by subcutaneous injection ofⅡcollogen. The expression of VEGF was detected with immunohistochemistry in different times. Results The incidence of arthritis in the rats immunized with CⅡwas approximately 88.57%. The arthritis index of the model group was rising, but that of the BZX decoction group and the methotrexate (MTX) group were decreasing on the 30th day. No expression of VEGF was found in the synovial membrane of the normal group. The expression of VEGF of the model group was notably higher than that of the BZX decoction group and the MTX group in different times (P<0. 01). The expression of VEGF of the model group rose step by step,but that of the BZX decoction group and the MTX group decreased and was significantly lower than that of the model group (P<0.01). The expression of VEGF of the BZX decoction group was significantly lower than that of the MTX group(P<0.05). Conclusion The expression of VEGF is relative to the synovial pannus formation in RA. BZX decoction and MTX could decrease the expression of VEGF, but the curative effect of BZX decoction is significantly better than that of MTX.BZX decoction could inhibit the formation of the synovial pannus or bone in RA by decreasing the expression of VEGF.