不同工艺提取的龙血竭中龙血素A、B的含量的测定

Determination of loureirin A and B extracted from Sanguis Draxonis in two extract processed by RP-HPLC

目的 :测定不同提取工艺提取的龙血竭中龙素A和龙血素B的含量。方法 :采用HPLC法 ,以C18反相键合硅胶为固定相 ,乙腈 - 1%冰醋酸 (34∶6 6 )为流动相 ,检测波长为 2 80nm ,用外标法定量。结果 :龙血素A在 98～ 4 90ng范围有良好线性关系 ,r=0 .9996 ,方法平均回收率为 97.4 5 % ,RSD为 1.82 %。龙血素B在 4 3.2～ 2 5 9.2ng范围有良好线性关系 ,r =0 .9993,方法平均回收率为 96 .92 % ,RSD为 1.5 7%。免加热工艺提取的龙血竭中龙血素A和龙血素B含量匀高于传统工艺提取的血竭。结论 :免加热工艺为一种独创的、提取率高的新技术 。

Objective: To establish HPLC method for the determination of loureirin A and loureirin B in Sanguis Draxonis Capsules.Methods: HPLC system included C 18 reverse phase column and acetonitrile 1% acetic acid (34∶66) as mobile phase, detection at 280nm and external standard method.Results:The standard curves of loureirin A was linear in the concentration range of 98～490ng, r =0.9996.The average recovery was 97.45%, RSD was 1.82%.The standard curves of loureirin B was linear in the concentration range of 43.2～259.2, r = 0.9993 .The average recovery was 96.92%, RSD was 1.57%.The contents of loureirin A and loureirin B in Sanguis Draxonis from heating free technology were higher than traditional technology.Conclusion: This heating free technology is a new technique that is a creation with higher extract rate,this method is worth populariging.