人胃癌组织中突变型P53蛋白的积聚与多重耐药基因的表达

The relationship between mutant P53 accumulation and MDR-1 gene expression in gastric cancer

目的 研究突变型P53蛋白在胃癌组织中的积聚对多重耐药基因的作用。方法 采用免疫组化法从蛋白水平检测胃癌组织的P53蛋白与多重耐药基因产物P-糖蛋白 (Pg蛋白 )水平。结果 46份胃癌组织标本中P53蛋白阳性率为60.87% ,Pg蛋白阳性率为73.91 %。在28份P53蛋白阳性表达中有23份 (82.15% )Pg蛋白表达阳性;18份P53蛋白阴性表达中9份 (50.00 % )为Pg蛋白表达阳性。P53蛋白和Pg蛋白表达阳性率具有相关性 (P<0.05) ,且P53蛋白、Pg蛋白表达与癌组织的分化类型亦有关。结论 P53蛋白在胃癌组织中的积聚与Pg蛋白异常表达有关 ,P53可能通过各种机制调控多重耐药基因的表达。

Objective To explore the relationship between mutant P53 and MDR-1 gene expression in gastric cancer.Methods Forty six surgical specimens of gastric cancer were examined the levels of P53 protein and p-glycoprotein(PgP) by immunohistochemical technique.Results In this study 28 of 46 samples (60.87%) were positive for P53, and 34 of 46 (73.91%) were positive for PgP.Pgp expression were detected in 23 of 28 P53-positive specimens, and in 9 of 18 P53-negative ones.There was a close correlation between P53 and PgP expression (P<0.05).And the expression of PgP and P53 was closely associated with histological differentiation of the carcinoma.Conclusion The results demonstrate that PgP expression is closely associated with P53 protein accummulation in human gastric cancer.