Weel蛋白在鼻咽癌组织中的表达及其与临床预后的关系

Expression of Wee1 protein in nasopharyngeal carcinoma and its relationship with clinical prognosis

目的探讨Wee1蛋白在鼻咽癌组织中的表达及其与临床预后的关系。方法收集2005年1月至2010年12月期间在张家口市第一人民医院和中国人民解放军251医院耳鼻喉科住院患者70例,手术病理活检均明确诊断为鼻咽癌,应用免疫组织化学(En VisionTM)法检测其Wee1蛋白表达,并与30例正常组织的表达进行比较,同时分析Weel蛋白的表达与鼻咽癌临床病理因素的关系及其5年生存率。结果 Wee1蛋白在鼻咽癌组织、正常组织中的阳性表达率分别为52.86%(37/70)、10.00%(3/30),两组的阳性表达率比较差异有显著统计学意义(P>0.01);Wee1蛋白的阳性表达与肿瘤直径、淋巴结转移、浸润邻近组织及EBV感染有关(P>0.05),与性别、年龄、组织学类型及肿瘤部位无关(P>0.05);Wee1蛋白阳性表达的鼻咽癌患者5年生存率为35.14%(13/37),明显低于Wee1蛋白阴性表达的81.82%(27/33),两组比较差异有显著统计学意义(P>0.01)。结论 Wee1蛋白的异常表达不仅参与了鼻咽癌的发生,而且有利于癌细胞的浸润转移,对预测患者的生存预后也有参考价值。

Objective To investigate the expression of Wee1 protein in nasopharyngeal carcinoma and its relationship with clinical prognosis. Methods Seventy patients with nasopharyngeal carcinoma were collected from the First People`s Hospital of Zhangjiakou City and the 251 thHospital of Chinese People’s Liberation Army from January 2005 to December 2010, which were definitely diagnosed as nasopharyngeal carcinoma by surgery pathology biopsy. The expression of Wee1 protein was detected by immunohistochemisty(En VisionTM) in nasopharyngeal carcinoma tissues in the70 patients and compared with that of normal tissues(n=30). The relationship between the expression of Wee1 protein and clinicopathological factors, the patients’ five-year survival rate were analyzed by χ2test. Results The positive expression rate of Wee1 protein was 52.86%(37/70) in nasopharyngeal carcinoma tissues and 10.00%(3/30) in normal tissues, with statistically significant difference(P<0.01). The positive expression of Wee1 protein in nasopharyngeal carcinoma tissues was related to tumor size, lymph node metastasis, infiltrating adjacent tissues and EBV infection(P<0.05), but not related to gender, age, histological category and tumor site(P>0.05). The five-year survival rate of the patients with nasopharyngeal carcinoma with the positive expression of Wee1 was 35.14%(13/37), which was significantly less than that of the patients with negative expression of(81.82%)(27/33), and the difference was statistically significant(P<0.01). Conclusion The overexpression of Wee1 not only takes part in the development of nasopharyngeal carcinoma, but also is useful for cancer infiltrating and metastasis, which is valuable in forecasting the survival and prognosis of patients.