摘要
目的探讨组织钙结合蛋白S100Al m RNA和锌指转录因子Snail m RNA检测在卵巢疾病中的临床应用。方法选取2013年3月至2015年3月我院经病理确诊的卵巢病变组织标本共68例,通过RT-PCR技术检测卵巢良性囊腺瘤(良性肿瘤组)、交界性囊腺瘤(交界性肿瘤组)和卵巢囊腺癌(卵巢癌组)组织S100Al m RNA和Snail m RNA的表达,并测定各组血清癌胚抗原(CEA)、糖类抗原125(CA125)及人附睾分泌蛋白(HE4)水平,比较三组患者以上指标的变化。结果卵巢癌组组织S100Al m RNA和Snail m RNA的表达量与交界性肿瘤组和良性肿瘤组比较明显增高,差异均有显著统计学意义(P<0.01),交界性肿瘤组与良性肿瘤组比较明显增高,差异也有显著统计学意义(P<0.01);卵巢癌和交界性肿瘤组S100Al m RNA和Snail m RNA相对表达量具有相关性(r=0.629、0.571,P<0.01),组织S100Al m RNA和Snail m RNA分别与血清CA125及HE4呈现相关性(r=0.632、0.587,P<0.01;r=0.638、0.617,P<0.01)。结论联合检测组织S100Al m RNA和Snail m RNA在卵巢良性囊腺瘤、交界性囊腺瘤和卵巢囊腺癌病变组织中的表达,可增加卵巢囊腺癌的早期检出率,为上皮性卵巢疾病的鉴别诊断提供新的思路。
Objective To explore the application of detecting S100 Al m RNA and Snail m RNA in tissues in the patients with ovarian disease. Methods A total of tissue specimens from ovarian disease diagnosed by pathological section were selected from March 2013 to March 2015. The expression of S100 Al m RNA and Snail m RNA in tissues was detected in the ovarian benign cystic adenoma(benign tumor group), borderline cystic adenoma(borderline tumor group) and ovarian cystadenocarcinoma(ovarian cancer group) by RT-PCR technique, as well as the levels of serum carcino-embryonic antigen(CEA), carbohydrate antigen 125(CA125), and human epididymis secretory protein 4(HE4).The changes of the above indicators were compared among the three groups. Results The expression rates of S100 Al m RNA and Snail m RNA were significantly higher in ovarian cancer group than borderline tumor group and the benign tumor group(P<0.01), and also in borderline tumor group than benign tumor group(P<0.01). The relative expressions of S100 Al m RNA and Snail m RNA in tissues were correlated in the ovarian cancer group and the borderline tumor group(r=0.629, 0.571, P<0.01), and the two markers were correlated with the levels of serum CA-125 and HE4(r=0.632,0.587, P<0.01; r=0.638, 0.617, P<0.01). Conclusion Joint detection of expression of S100 Al m RNA and Snail m RNA in tissues in the ovarian benign cystic adenoma, borderline cystic adenoma and ovarian cystadenocarcinoma can increase the early detection rate of ovarian cystadenocarcinoma, providing a new thought for the differential diagnosis of the ovarian diseases.
出处
《海南医学》
CAS
2016年第7期1051-1054,共4页
Hainan Medical Journal