海南三叉苦抗炎作用及机制研究

Anti-inflammatory effect and mechanism of Evodia lepta in Hainan

目的研究海南三叉苦水提物的抗炎作用并探索其作用机制。方法通过二甲苯致小鼠耳肿胀、角叉菜胶致大鼠足肿胀来观察三叉苦的抗炎作用。采用酶联免疫吸附法(ELISA法)测定肿瘤坏死因子α(TNF-α)和白细胞介素1β(IL-1β)的含量。结果与模型组(0)相比,三叉苦水提物中、高剂量(19.11%,22.94%)能抑制二甲苯所致小鼠耳肿胀(P<0.05),中剂量对角叉菜胶致大鼠足肿胀有明显的抑制作用,在致炎4 h[(0.53±0.17)cm]效果最明显(P<0.01)。水提物中、高剂量对大鼠血清中TNF-α[(169.61±73.80)ng/L和(124.34±85.65)ng/L]和IL-1β[(521.01±206.89)ng/L和(393.99±197.61)ng/L]有不同程度的降低作用,TNF-α较模型组[(283.98±68.68)ng/L]降低显著(P<0.05)。结论海南三叉苦水提物具有抗炎作用,其抗炎作用可能与抑制TNF-α和IL-1β的生成有关。

Objective To study the anti-inflammatory effect and mechanism of Evodia lepta in Hainan.Methods The anti-inflammatory effect of Evodia lepta was observed by acute inflammation model of the mouse ear swelling and rat foot swelling. The contents of tumor necrosis factor alpha(TNF-α) and interleukin 1 beta(IL-1 β)were determined by enzyme-linked immunosorbent assay(ELISA). Results Compared with the model group, the middle and high dose water extracts(19.11%, 22.94%, respectively) of Evodia lepta could inhibit the ear swelling induced by xylene(P<0.05); The water extracts at middle doses could remarkably restrained paw edema induced by carrageen, with the best anti-inflammatory effect at 4 h [(0.53±0.17) cm, P<0.01]; The water extracts at middle and high dose could reduce the content of TNF-α [(169.61±73.80) ng/L vs(124.34±85.65) ng/L] and IL-1β [(521.01±206.89)ng/L vs(393.99±197.61) ng/L] in rat serum; Compared with the model group [(283.98±68.68) ng/L], the TNF-α was decreased significantly(P<0.05). Conclusion The water extract of Evodia lepta has anti-inflammatory effect, and its anti-inflammatory effect may be related to inhibiting the formation of TNF-α and IL-1β.