盘状红斑狼疮患者T淋巴细胞CD70基因的表达及临床意义

Expression of CD70 in T lymphocytes from patients with discoid lupus erythematosus and its clinical significance

目的检测盘状红斑狼疮(DLE)患者外周T淋巴细胞CD70基因表达水平,并探讨CD70基因在DLE发病机理中的作用。方法 RT-PCR测定2014年1月至2015年6月在我院就诊的15例活动期、15例非活动期DLE患者和同期健康体检的15例健康人(对照组)外周血T淋巴细胞CD70 m RNA转录水平,流式细胞术检测各组样本中CD4+CD70+T/CD8+CD70+T的阳性率。结果活动期和非活动期DLE患者T淋巴细胞中的CD70 m RNA转录水平分别为(4.5±0.19)和(2.9±0.09),显著高于对照组的(1.9±0.11),差异均有显著统计学意义(P<0.01),且活动期DLE患者T淋巴细胞中的CD70 m RNA的转录水平明显高于非活动期DLE患者,差异具有统计学意义(P<0.05);活动期和非活动期DLE患者的外周血CD4+CD70+T淋巴细胞的阳性率分别为(18.82±1.22)%和(15.32±1.01)%,明显高于对照组的(14.63±0.41)%,差异均有统计学意义(P<0.05或P<0.01),且活动期DLE患者的阳性率显著高于非活动期DLE患者,统计有显著统计学意义(P<0.01);活动期和非活动期DLE患者T淋巴细胞中CD8+CD70+的阳性率与对照组比较,差异均无统计学意义(P>0.05)。结论 CD70基因的过度表达在DLE的发生发展中起着重要的作用,且CD70过度表达可作为检测DLE疾病患者的一个重要指标。

Objective To investigate the expression of CD70 in T lymphocytes of discoid lupus erythematosus(DLE) patients and the role of CD70 in DLE pathogenesis. Methods Blood samples were obtained from 15 patients with active DLE, 15 patients with unreactive DLE and 15 normal human controls from January 2014 to June 2015. Quantitative RT-PCR and flow cytometry were applied to detect the transcriptional level of CD70 m RNA and the positive rate of CD4+CD70+T/CD8+CD70+T cells respectively. Results The levels of CD70 m RNA in T lymphocytes in active DLE(4.5±0.19) and non-active DLE group(2.9±0.09) were significantly higher than that in the normal control group(1.9±0.11)(P<0.01). Moreover, the levels of CD70 m RNA in the active DLE group was significantly higher than that in non-active DLE group(P<0.05). The positive rate of peripheral blood CD4+CD70+T cells in active DLE group[(18.82±1.22)%] and non-active DLE group [(15.32±1.01)%] were significantly higher than that in the normal control group [(14.63±0.41)%](P<0.05, P<0.01). The active DLE group showed higher positive rate than non-active DLE group(P<0.01). However, there was no significant difference between the three groups in the positive rate of CD8+CD70+T cells(P>0.05). Conclusion This study shows that over-expression of CD70 plays a significant role in the pathogenesis of DLE and could be used as an important index of disease detection in patients with DLE.