慢性心力衰竭患者血浆miRNA表达谱分析

Profiling of plasma miRNA in chronic heart failure patients

目的对慢性心力衰竭(CHF)患者的血浆miRNA进行二代测序,探讨CHF患者血浆中miRNA的表达差异。方法收集2013年7月至2015年6月间在北京大学深圳医院治疗的40例CHF患者和10例健康对照个体,应用第二代高通量测序技术对其血浆miRNA进行测序,寻找差异表达的miRNA。另收集同期CHF患者和健康对照个体各96例,用逆转录-实时荧光定量聚合酶链反应对差异表达的miRNA进行验证。结果与健康对照者相比,高通量测序显示在CHF患者的血浆中miR-184、miR-651-5p、miR-130b-5p、miR-203a-3p、miR-548e-3p和miR-106a-5p的表达水平存在显著上调,miR-31-5p和miR-5091的表达水平存在下调,逆转录-实时荧光定量聚合酶链反应检测血浆miRNA的表达差异与高通量测序的结果相符。结论 CHF患者的血浆miRNA与健康个体的表达谱存在差异,差异表达的miR-184、miR-651-5p、miR-130b-5p、miR-203a-3p、miR-548e-3p、miR-106a-5p、miR-31-5p和miR-5091或许与CHF的发生有关。

Objective To search for differentially expressed plasma miRNAs between patients with chronic heart failure(CHF) and healthy controls by next-generation small RNA sequencing. Methods Forty CHF patients and10 healthy volunteers were recruited to search for differentially expressed plasma miRNAs in next-generation small RNA sequencing study from Peking University Shenzhen Hospital between July 2013 and June 2015. miRNAs expression were validated in another 96 patients and 96 controls by RT-PCR analysis during the same period. Results Comparing to healthy group, miRNA sequencing showed that miR-184, miR-651-5p, miR-130b-5p, miR-203a-3p,miR-548e-3p, and miR-106a-5p were upregulated and miR-31-5p and miR-5091 were downregulated in CHF patients. The levels of miRNA measured by RT-PCR were consistent with the results obtained from miRNA sequencing technology. Conclusion A differentially expressed miRNA profiling was seen between patients and healthy individuals.The miR-184, miR-651-5p, miR-130b-5p, miR-203a-3p, miR-548e-3p, miR-106a-5p, miR-31-5p, and miR-5091 may be closely involved in the pathogenesis of chronic heart failure.