BC-6800血细胞分析仪白细胞分类复检规则的制定及应用评价

Establishment and clinical evaluation of hematology review criteria for differential leucocytes count of BC-6800 hematology analyzer

目的建立BC-6800血细胞分析仪复检规则,并评价其合理性。方法应用BC-6800血液分析仪检测400份血常规标本,同时每份标本制做两张血涂片,由从事形态学工作10年以上的两名检验人员进行人工镜检。计算真阳性率、真阴性率、假阳性率、假阴性率。结果人工镜检的结果与仪器的报警信息比较,阳性符合率为28%,假阳性率为8%,阴性符合率为65.5%,假阴性率为2.5%。本实验室制定的复检规则漏检率(假阴性率)为2.5%,符合国际血液学复审协作组关于假阴性率小于5%的规定,复检率为24%。结论我室将在检验工作中严格遵守这些复检规则,并根据临床需求进一步完善复检规则,降低漏检率和推片率。

Objective To establish the hematology review criteria for differential leucocytes count of BC-6800 hematology analyzer, and evaluate its rationality. Methods A total of 400 blood samples were detected with BC-6800 hematology analyzer. At the same time, two blood smears were produced for each specimen, and artificial microscopy was carried out by two inspectors who have 10 years of morphological inspection work experience. The true positive rate, true negative rate, false positive rate and false negative rate were calculated based on that. Results The comparison of the results of artificial microscopy and the instrument alarm information showed that the positive coincidence rate, the false positive rate, the negative coincidence rate and the false negative rate were 28%, 8%, 65.5%,2.5%, respectively. The missed rate of review criteria(false negative rate) established in our laboratory was 2.5%,which was in line with the standard of the false negative rate(<5%) of the International Collaborative Group with 24%re-examination rate. Conclusion We will strictly abide by these review criteria, further improve the criteria based on clinical need, and reduce the missed rate and re-examination rate.