一测多评测定玉叶金花中4种有机酸的含量

Simultaneous determination of four organic acids in Mussaendapubescensby QAMS

建立玉叶金花提取物中有机酸类成分含量测定的一测多评方法,探讨一测多评法在中药材质量控制中的应用.以绿原酸(3-咖啡酰奎宁酸)为内标物,分别建立异绿原酸A(3,5-二咖啡酰奎宁酸)、异绿原酸B(3,4-二咖啡酰奎宁酸)、异绿原酸C(4,5-二咖啡酰奎宁酸)的相对校正因子,通过外标法和一测多评法2种不同的方法测定计算4种有机酸的含量,并比较不同方法结果的相对误差.利用一测多评法和外标法测定广西6个不同产地的4种有机酸类成分结果无显著差异.一测多评可用于玉叶金花中4种有机酸类成分的测定,且具良好的可行性,同时具有较高的经济性.

A method of quantitative analysis of multi-components by single-marker for the determination of organic acid content in the extract of Mussaenda pubescens is established,the application of the method for the quality control of the traditional Chinese medicine is discussed.Chlorogenic acid(3-caffeoylquinic acid),was used as the internal standard to establish the relative correction factors of isochlorogenic acid A(3,5-O-di-caffeoylquinic acid),isochlorogenic acid B(3,4-O-di-caffeoylquinic acid),and isochlorogenic acid C(4,5-O-di-caffeoylquinic acid),respectively.The external standard method and QAMS method were used to calculate four kinds of relative correction factors.The content of organic acids were determined and the relative error of the results of the two methods was compared.There is no significant difference between the two methods for the determination of four organic acid components in six different producing areas in Guangxi.The method of QAMS can be used to determine the content of four organic acids in Mussaenda Pubescens,and it shows good feasibility and high economic efficiency.