小麦种质系山农10103抗白粉病基因的分子标记定位

Molecular Marker Localization of Powdery Mildew Resistance Gene in Wheat Germplasm Line Shannong 10103

为明确小麦种质系山农10103抗白粉病基因的遗传特点,利用山农10103与高度感染白粉病的小麦品种辉县红杂交构建F2分离群体,并利用分子标记技术对抗白粉病基因进行了染色体定位。结果表明,辉县红高度感染白粉病,山农10103和山农10103×辉县红F1对白粉病表现为免疫或近免疫,F2群体抗感分离符合3∶1的分离比例,证明山农10103的白粉病抗性由1对显性主效基因控制,暂将其命名为PML10103;在2 606对SSR、ETS-SSR和STS引物中,筛选得到7个与白粉病基因PML10103连锁的标记Xcinau188、XCAU127、CFE164、CWM109、SWES231、Xcnl113和SWES1015,它们与抗白粉病基因的连锁距离分别为0.4,0.7,4.8,3.0,4.9,3.3,0.9 c M;根据已发表的分子标记图谱,将PML10103定位在6B染色体上。

To identify the genetic characteristic of powdery mildew resistance gene in wheat genotype Shannong 10103,F2 segregation population derived from the cross between Shannong 10103 and Huixianhong was created and molecular marker analysis was carried out in this study.The results showed that Shannong 10103 and Shannong 10103 Huixianhong F1 were resistant to powdery mildew in seedlings,and the segregation ratio of resistant and sus-ceptible plants in F2 was 3∶1.All these results suggested that the powdery mildew resistance in Shannong 10103 was controlled by a dominant gene,named PML10103.Through screening 2 606 pairs of SSR,EST-SSR and STS primers for polymorphism in the parents and preferred group, seven SSR, EST-SSR markers ( Xcinau188, XCAU127, CFE164,CWM109,SWES231,Xcnl113 and SWES1015) were linked to PML10103.The genetic distance between the powdery mildew resistance gene and the SSR,EST-SSR markers-Xcinau188,XCAU127,CFE164,CWM109, SWES231,Xcnl113 and SWES1015 were 0.4,0.7,4.8,3.0,4.9,3.3,0.9 cM,respectively.Take the advantage of the known molecular linkage map,PML10103 was mapped on wheat chromosome 6B.