银蓝水貂血液蛋白多态性与经济性状相关性研究

Studies on Correlation of Blood Protein Polymorphism and Economic Character in Sliver Blue Mink

为了分析银蓝水貂血液蛋白多态性与其生长性状的关联性。利用不连续垂直板聚丙烯酰胺凝胶电泳对银蓝水貂血液中转铁蛋白(Tf)、抗胰蛋白酶(Pi-1、Pi-2)、维生素D结合蛋白(GC)3个蛋白位点进行检测,检测结果表明,GC没有多态性,Tf、Pi-1具有多态性(Pi-2分离效果不清晰),通过建立一般线性模型筛选银蓝水貂遗传标记,方差分析显示,Pi-1是对银蓝水貂体重影响较大的位点(P=0.013<0.05),Pi-1(P=0.029<0.05)和Tf(P=0.039<0.05)对银蓝水貂体长有显著影响,筛选出Pi-1 AA、Tf AC是银蓝水貂的高产基因型。为生化遗传标记辅助选择运用于银蓝水貂的选育提供了可靠的依据。

The aim of this study was to analyze the association of blood protein polymorphism with some eco-nomic traits in the sliver blue mink.Three blood protein loci of sliver blue mink breeds in China,which included protease inhibitor-1,protease inhibitor-2,Vitamin D binding protein(GC) and Transferrin(Tf) were detected by method of vertical polyacrylamide gelelectrophoresis .The results showed that GC loci in mink were monomorphic,Tf and Pi-1 loci were polymorphic,(Pi-2 was not detected clearly),General linear model was founded for the selection of biochemical genetic marker.Least squares variance analysis showed that there was significant difference for Pi-1 loci of sliver blue mink and weight(P =0.013 <0.05),Pi-1(P =0.029 <0 .05)、Tf(P =0.039 <0.05)loci of sliver blue mink and length.Pi-1 AA,Tf AC were the high yielding genetypes of sliver blue mink.For biochemical genetic marker assisted selection used in silver blue mink breeding to provide reliable basis.