83份早熟抗虫棉种质资源的SSR标记聚类分析

Cluster Analysis in 83 Early-maturing Cotton Germplasms by SSR Markers

早熟棉遗传多样性研究是早熟棉遗传改良的基础,对培育早熟棉新品种有重要意义。为了进一步改善现有早熟棉品种的早熟性,为早熟棉育种的亲本组配提供依据,利用35对SSR分子标记,对来自不同地区的83份早熟抗虫棉种质进行遗传多样性分析。结果表明,筛选出的22对多态性引物共扩增出30条多态性条带。NTSYS-pc2.11的遗传多样性分析表明,83份材料的相似系数变化为0.73~0.98,当遗传相似系数为0.73时,将83份棉花早熟材料分为两大类。第Ⅰ类群包含59个材料,第Ⅱ类群包含24个材料。表明我国早熟棉遗传基础比较狭窄,今后育种中要选用亲缘关系较远的材料作为杂交亲本,创制新的早熟棉种质资源。

It is very important to study the genetic diversity of early-maturing cotton for breeding early-maturing cultivar. To improve the prematurity of early-maturing cotton and select right parent combinations,we analysed the genetic diversity of 83 early-maturing cotton germplasms from different ecotypes in China using 35 pairs of SSR primer. As a result,we got 22 pairs of polymorphic primer and 30 allelic variations. The result of NTSYS-pc2. 11 analysis indicated that genetic similarity coefficient of the 83 germplasms ranged from 0. 73 to 0. 98,which showed that there was limited genetic diversity in early-maturing cotton germplasms. The 83 early-maturing cotton germplasms were divided into 2 groups with the genetic similarity coefficient at 0. 73,which included 59 and 24 members respectively. We should select parent combinations with distant phylogenetic relationships for early-maturing cotton breeding in future work.