甜瓜品种SSR指纹图谱的构建

Establishment of SSR Fingerprint on Melon Hybrids and Their Parents

为实现对甜瓜品种快速准确鉴定,利用SSR分子标记技术对6个甜瓜品种及其亲本共18份材料构建指纹图谱。通过利用50对多态性好的甜瓜SSR引物,经扩增电泳分析得到C17、C21、C30共3对引物,可用于构建18份材料的指纹图谱,实现了对这些材料的分子鉴定。另外利用C21引物对材料M324的杂种品种进行纯度鉴定,结果纯度为89%,与田间性状统计结果 90%相符。

For the purpose of melon identification,SSR technology was used to establish fingerprint for 18 melon accessions included six F1 hybrids and their parents. Based on 50 SSR primer pairs were assessed validation of the amplification in above melon accessions. Used three primer pairs C17,C21 and C30,SSR fingerprint was obtained for six melon F1 hybrids and their parents,which was able to distinguish all of the 18 accessions. Moreover,primer C21 was applied in purity identification of M324 hybrid,the result indicated that the purity was 89%,which were approximate to their field purity( 90%).