草莓分子指纹图谱构建与应用

Establishment and Application of Strawberry Molecular Fingerprinting

为了对草莓品种进行分子鉴定,以草莓品种红颊、章姬、宁馨(香莓)、阿尔比和梦香为试验材料,从112个RAPD引物中筛选出4个差异引物,扩增出5个差异位点进行指纹图谱构建和聚类分析。RAPD揭示供试草莓材料的多态性比例为3.6%。应用指纹图谱标记能够鉴定红颊、阿尔比和梦香等推广品种,构建宁玉和香蕉等新增草莓品种的指纹图谱,发现梦香的变异群体。最后讨论了草莓指纹图谱的适用性和品种聚类分析对制定推广计划的指导作用。

To investigate the strawberry cultivars by molecular markers,Hongjia,Zhangji,Ningxin(Xiangmei), Aerbi and Mengxiang were used to establish the molecular fingerprinting and cluster analysis by RAPD markers. Four markers were screened from 112 RAPD,which generated five polymorphic loci.These RAPD markers showed the polymorphism ratio of tested strawberry varieties were 3.6%.Hongjia,Aerbi and Mengxiang could be verified, the fingerprinting of Ningyu and Xiangjiao could be established and the variation of Mengxiang population could be detected by fingerprinting markers.Finally,the applicability of strawberry molecular fingerprinting and the certain instructive effect on cultivars spreading of cluster analysis were also discussed.