小麦内生细菌W-1的分离筛选及其对小麦纹枯病菌的抑制作用

Screening of Wheat Endophytic Bacteria W-1 and the Inhibition to Rhizoctonia cerealis

小麦纹枯病是小麦生产上的重要病害、难防病害之一,寻找安全高效防治途径是控制该病害的一项任务。内生细菌的筛选与应用是生物防治中的热点之一,为筛选获得对小麦纹枯病菌有拮抗作用的内生细菌,丰富小麦纹枯病的防治方法,采用涂布平板法从小麦植株中分离内生菌,并通过抑菌圈法和平板对峙培养法筛选得到对小麦纹枯病菌有明显抑制作用的菌株。结果表明,分离得到的8株内生菌菌株中,有4株菌株对小麦纹枯病菌有拮抗作用,其中W-1的抑菌效果最好;用其发酵液处理小麦纹枯病菌,导致菌丝形态畸形、消融,甚至断裂;发酵液处理的小麦生长正常,且该菌株可以在小麦植株中稳定定殖。

Wheat sharpeye sport caused by Rhizoctonia cerealis is very important and difficult control disease in wheat production. Exploring new safely,high efficiency control method is an important test in this disease control.Screening and applying the endophytic bacteria is an important focus in biological control of plant disease. In order to acquire endophytic bacteria against R. cerealis and enrich the methods of control wheat sharp eyespot,the endophytic bacteria strains from wheat were isolated by using spread plate method. The antagonistic strains were screened by inhibition zone method and tablet confrontation culture method. The results showed that 4 of 8 plants endophytic bacteria strains isolated from the wheat displayed antagonistic activity to Rhizoctonia cerealis,and W-1 was the best one. The mycelia of Rhizoctonia cerealis were treated with fermentation liquid of W-1 and showed deformity,ablation and fracture. However,the wheat could grow normally treated with fermentation liquid of W-1. Colonization test showed that the isolate W-1 could colonize in wheat stably.