大豆广谱抗源对SMV优势株系的抗性遗传和等位性分析

Inheritance and Allelism Analysis of Resistance to Soybean mosaic virus Predominant Strains in Soybean

为了研究大豆广谱抗源对我国大豆花叶病毒优势株系SC3和SC7的遗传方式及抗源材料对SMV抗性基因间的等位性关系,利用广谱抗源科丰1号和齐黄1号与感病材料南农1138-2配制抗感及抗抗杂交组合,通过人工摩擦接种法进行鉴定。结果发现,接种株系SC3和SC7后,科丰1号和齐黄1号与南农1138-2配制抗感组合的F1均表现抗病,经卡方测验,F2抗感分离比例符合3∶1,F2∶3家系分离比例为1(抗)∶2(分离)∶1(感),说明这2个广谱抗源均有1对显性基因控制株系SC3和SC7的抗性;等位性测验结果表明2个抗抗组合的F1对SC3和SC7优势株系均表现抗病,F2分离比符合15(抗)∶1(感),说明科丰1号和齐黄1号对株系SC3和SC7的抗性基因不等位且独立遗传。进一步分析2个广谱抗源携带的抗性基因可以发现,科丰1号对株系SC3的抗性基因RSC3和齐黄1号对SC7株系的抗性基因RSC7Q可能位于大豆的2号和13号染色体上,为利用大豆广谱抗源进行抗SMV育种奠定了很好的基础。

In order to research the resistance the relationship among the resistance genes present in these two broad-spectrum resistance cultivars to Soybean mosaic virus( SMV) predominant strains SC3 and SC7 in China. Two broad-spectrum soybean resistant( R) cultivars Kefeng No. 1 and Qihuang No. 1 were crossed respectively with susceptible( S) cultivars Nannong 1138-2 and resistant parent was also crossed with each other. The experiments were performed by using the artificial friction way under greenhouse/net-house conditions. The F2 and F2 ∶ 3lines derived from the R × S exhibited a segregation pattern of 3R∶ 1S and 1R∶ 2segregating∶ 1S ratio respectively when inoculated with the SMV strains SC3 and SC7. The results indicated that Kefeng No. 1 and Qihuang No. 1 carried single dominant resistant gene for both SC3 and SC7. The test of the allelism of the resistance genes to SC3 and SC7 showed that the F2 from R × R crosses exhibited a segregation pattern of 15R∶ 1S. The results suggested that the resistance genes between Kefeng No. 1 and Qihuang No. 1 were not at the same locus. Additionally,we inferred that Kefeng No. 1 carried the RSC3 gene maybe on chromosome 2 and Qihuang No. 1 carried the RSC7 Qgene on chromosome 13,these results are important for soybean resistance breeding programs.