黄连生物碱4种提取方法的比较

Comparative Study of 4 Different Extraction Methods for Alcaloids from Rhizoma Coptidis

采用超声波法、回流法、冷浸法和酶辅助冷浸法提取黄连生物碱,采用高效液相色谱法对提取液中的生物碱含量进行检测。结果表明,超声波法、回流法、冷浸法和酶辅助冷浸法的黄连小檗碱提取率分别为26.10%、52.24%、54.15%和81.27%;总生物碱提取得率分别为28.66%、59.70%、61.58%和89.36%。酶辅助冷浸法提取黄连生物碱所得提取率较高,优于其他3种提取工艺。

Ultrasonic method,reflux method,cold-soaking method and enzyme-assisted cold-soaking method were used to extract alkaloids from Rhizoma Coptidis;and HPLC was used for quantitative analysis of alkaloids.The results indicated that the yield of berberine of ultrasonic extraction,reflux method,cold-soaking method and enzyme-assisted cold-soaking method was 26.10%,52.24%,54.15% and 81.27%,respectively.And the yield of total alkaloids of the 4 methods was 28.66%,59.70%,61.58% and 89.36%,respectively.The extraction yield of alkaloids of enzyme-assisted cold-soaking method was higher than other three extraction methods.