牛大力离体培养与植株再生条件的优化

Study on in vitro Culture and Plant Regeneration of Milletia speciosa

分别以野生牛大力(Milletia speciosa)成熟种子、带芽茎段为材料研究不同激素组合及外植体的离体培养效果。结果表明,1 g/L HgCl2对牛大力种子和带芽茎段分别消毒10 min和15 min效果最好;愈伤组织和不定芽诱导的最佳激素组合为1.5 mg/L 6-BA+0.5 mg/L NAA+0.3 mg/L IBA,种子和带芽茎段外植体的最高不定芽诱导率分别为95.8%和45.8%;最适合不定芽分化的激素组合为2.0 mg/L 6-BA+0.5mg/L NAA,最高分化率为58.3%;最佳的生根培养基为1/2MS+1.5 mg/L NAA+1.0 mg/L IBA,生根率高达70.8%;最佳移栽基质为等体积混合的黄土、泥炭和河沙,移栽90 d后成活率为73.3%。

Using seeds and stems with buds of wild Milletia speciosa as material,the effects of different hormone combination and explants on the in vitro culture were investigated.The result showed that the optimum treatment time of 1 g/L HgCl2 for seed and stem was 10 and 15 min respectively.The best hormone combination for callus and adventitious buds induction was 1.5 mg/L 6-BA+0.5 mg/L NAA+0.3 mg/L IBA,the highest callus induction rate of seeds and adventitious buds of stem with a bud was 95.8% and 45.8%,respectively.The best medium for differentiation of adventitious bud was 2.0 mg/L 6-BA +0.5 mg/L NAA;the differentiation rate was 58.3%.And the best rooting medium was 1/2MS+1.5 mg/L NAA+1.0 mg/L IBA;the rooting rate was 70.8%.The best matrix was mixture of equivalent volume of loess,peat and river sand,the survival rate of 90 days after transplantation reached 73.3%.