摘要
采用水提醇沉法提取苦瓜(Momordica charantia L.)中的多糖,在单因素试验的基础上设计正交试验考察提取过程中提取温度、提取时间、料水质量比和V浸提液∶V乙醇对多糖提取率的影响,并对所提取苦瓜多糖的抗氧化活性进行了检测。结果表明,最佳提取工艺为提取温度90℃、提取时间2 h、料水质量比1∶35、V浸提液∶V乙醇=1∶4.0。苦瓜多糖对1,1-二苯基-2-三硝基苯肼自由基(DPPH.)和超氧阴离子自由基(O2-.)有较好的清除作用,在试验范围内清除率随苦瓜多糖用量的增加而升高。
Using water extraction and alcohol precipitation method,polysaccharide was extracted from fruit of Momordica charantia L..The effects of extraction temperature,treatment time,mass ratio of material to water and Vextraction solvent∶Vethanol on polysaccharide yield were studied by single factor tests,based on which orthogonal design was conducted to optimize the extraction technology.The anti-oxidation activity of obtained polysaccharide was detected.The results showed that the optimal extracting conditions were extracting at 90 ℃ for 2 h with mass ratio of material to water at 1∶35 and Vextraction solvent∶Vethanol=1∶4.0.Polysaccharide extracted from M.charantia L.could scavenging DPPH· and O2-· efficiently;and the scavenging rate increase with the increasing of polysaccharide dose.
出处
《湖北农业科学》
北大核心
2012年第24期5761-5763,5768,共4页
Hubei Agricultural Sciences
基金
龙岩学院大学生创新性实验计划项目(201137)
