黄豆幼苗生长过程中第一真叶与第二真叶生理指标的比较

Comparision of physiological indexes of the first leaf and second leaf in the growth of soybean seedlings

以黄豆[Giycine max (Linn.) Merrill]幼苗的第一真叶和第二真叶为研究对象,测定了第一真叶和第二真叶的可溶性蛋白质、光合色素、丙二醛和抗坏血酸含量以及过氧化氢酶和过氧化物酶活性。结果表明,第一真叶与第二真叶中的可溶性蛋白质含量没有显著性差异;第二真叶中的叶绿素a和类胡萝卜素含量高于第一真叶,分别高23.4%和89.9%,第一真叶中的叶绿素b含量高于第二真叶,高76.9%,总光合色素含量在第一真叶与第二真叶中较为接近,第二真叶中叶绿素a/b的值是第一真叶中的2.18倍;第一真叶的丙二醛含量高于第二真叶,前者是后者的3.04倍;第二真叶的抗坏血酸含量高于第一真叶,是后者的1.52倍;两真叶中未测到过氧化物酶和过氧化氢酶活性。抗坏血酸含量与可溶性蛋白质含量、丙二醛含量和叶绿素b含量为负相关,与叶绿素a含量和类胡萝卜素含量为正相关。

Soluble protein content, photosynthetic pigment content, MDA content, ascorbic acid content, enzyme activity and peroxidase activity of hydrogen peroxide in the first leaf and the second leaf of soybean [Giycine max(Linn.) Merrill] seedlings were determined. The results showed that there was no significant difference in soluble protein content between the first and second true leaf. The content of chlorophyll a and carotenoid in the second leaf was 23.4% and 89.9% higher than those in the first leaf, respectively. The content of chlorophyll b in the first leaf was 76.9% higher than that in the second leaf. The total photosynthetic pigment content in first leaf and second leaf were close to each other. The value of chlorophyll a/b in the second leaf was about 2.18 times higher than that in the first true leaf. The MDA content in the first true leaf was 3.04 times higher than that in the second true leaf. The content of ascorbic acid in the second leaf was 1.52 times higher than that in the first true leaf. The peroxidase activity and catalase activity could not been determined in these two leaves. Besides that, ascorbic acid content was negatively correlated with the content soluble protein, MDA and chlorophyll b, and was positively correlated with the content of chlorophyll a and carotenoid.