人参皂苷Rb1对肠缺血再灌注致肾损伤的保护作用

Ginsenoside Rb1 Attenuates Intestinal Ischemia-Reperfusion Induced Renal Injury

目的:研究人参皂苷Rb1对肠缺血再灌注(IIR)致肾损伤的保护机制。方法:成年雄性C57BL/6J小鼠随机分为5组:(1)假手术组(sham组);(2)模型组(IIR组);(3)生理盐水组(NS组);(4)低剂量组(Rb1-30组);(5)高剂量组(Rb1-60组)。于再灌注2h时取各组肾脏组织观察肾脏病理学形态并行评分;同时检测肾脏组织SOD和MDA含量;肾脏TUNEL细胞凋亡检测;以及免疫组化和Western Blot分析Caspase-3含量。结果:IIR组中肾脏病理学评分较sham组明显升高(P<0.01);肾脏组织中MDA含量增加,SOD活性降低(P<0.01)。Rb1-30组和Rb1-60组中肾脏病理学评分较IIR组明显降低(P<0.01);肾脏组织中MDA含量降低,SOD活性升高(P<0.01)。IIR组中TUNEL阳性细胞率和Caspase-3蛋白含量较sham组升高(P<0.01);Rb1-30组和Rb1-60组中TUNEL阳性细胞率和Caspase-3蛋白含量较IIR组降低(P<0.01)。结论:肠缺血再灌注可引发肾脏损伤;人参皂苷Rb1通过减轻肾脏细胞凋亡对其损伤起到保护作用。

Objective:To investigate the effects of ginsenoside Rb1 on intestinal ischemia reperfusion(IIR)induced renal injury in mice.Methods:Adult male C57 BL/6 Jmice were randomly divided into five groups:(1)sham group;(2)IIR group;(3)saline reperfusion(NS group);(4)30 mg/kg ginsenoside Rb1(Rb1-30)group;and(5)60 mg/kg ginsenoside Rb1(Rb1-60)group.Renal histology was observed and evaluated.Renal SOD and MDA levels were detected.In addition,renal tissue TUNEL and Caspase-3 expressions were performed.Results:IIR induced renal injury,which was characterized by increase of histological severity score and renal MDA level,while renal SOD level was decreased.Ginsenoside Rb1(30,60 mg/kg)lightened renal injury,that was in parallel with the decreased TUNEL-positive cells and Caspase-3 expressions.Conclusion:Ginsenoside Rb1 attenuates renal injury induced by IIR through attenuating apoptosis.