大鼠皮肤伤口感染模型的建立

Establishment of Skin Wound Infection Model in Rats

目的:探讨大鼠皮肤伤口大肠杆菌(E.coli)和金黄色葡萄球菌(S.aureus)感染模型的制备方法,建立大鼠背部皮肤伤口感染的模型,为临床创伤及术后伤口感染愈合提供小动物研究模型,同时为新型生物医学敷料及药物等的研究提供模型基础。方法:剃除大鼠背部毛发,同时用7%硫化钠溶液对大鼠背部剩余的毛发彻底清除。于大鼠脊柱两侧分别剪取直径为1.5cm的伤口,保证伤口深达肌层。将实验分为3组:(1)非感染组;(2)E.coli感染组;(3)S.aureus感染组。在大鼠行伤口制备术后,按分组将100μl生理盐水或菌液滴加到相应组别伤口表面。在伤口表面贴附临床静脉留置针贴膜,同时在贴膜表面加以医用胶布固定。术后每只大鼠于独立通气笼具(IVC笼具)内饲养,24-48h后观察各组伤口表面感染情况。结果:术后48h伤口处可形成明显的感染灶,E.coli菌液浓度在109集落形成单位(CFU)/ml以内可形成明显感染且不致死,而S.aureus感染伤口菌液浓度在超过10~8 CFU/ml后感染严重且致死率较高,菌液浓度控制在106-10~8 CFU/ml之间能够形成明显的S.aureus感染灶且能保证大鼠存活。结论:S.aureus菌液(10~6-10~7 CFU/ml,100μl)、E.coli菌液浓度(107-10~8 CFU/ml,100μl)能够成功制备大鼠伤口感染模型,且保证大鼠正常存活。

Objective:To explore the preparation method of rat skin wounds of E.coli and S.aureusinfection model,establish the model of back skin wound infection in rats,provide healing small animal models of wound infection after trauma and operation,and provide the model foundation for research of new biomedical dressing and drugs,etc.Methods:The experiment was divided into three groups:(1)non infection group;(2)E.coli infection group;(3)S.aureus infection group.After the rats were given wound preparation,100μl saline or bacterial fluid was added to the wound surface of the corresponding group by grouping.Results:Postoperative wound infec-tion was shown after 48 hours.The optimal concentration to induce infection was no more than109 CFU/ml and 10~8 CFU/ml for E.Coli and S.aureus with high survival rate.Conclusion:S.aureus 10~6-10~7 CFU/ml(100μl)and E.coli 100μl(107-10~8 CFU/ml)can successfully prepare the wound infection model in rats,and ensure the normal survival of rats.