TET1在肝癌组织中的表达及临床意义

Expression and Clinical Significance of TET1 in Hepatocellular Carcinoma

目的:观察肝细胞癌组织中10-11转位酶1(TET1)的表达水平,并探讨其与临床病理特征间的关系及临床意义。方法:应用定量RT-PCR及组织芯片免疫组化技术检测126例肝细胞癌(HCC)及癌旁组织中TET1的mRNA、蛋白表达水平,分析其与临床病理特征间的关系。结果:HCC中TET1mRNA表达相对强度[0.07(0.01,0.14)]较癌旁组织[1(1,1)]明显下降(P<0.05);HCC中77.0%(97/126)TET1蛋白低表达,而癌旁组织中72.2%(91/126)TET1呈高表达,HCC中TET1蛋白的表达水平明显低于癌旁组织(P<0.05);HCC中TET1蛋白表达水平与性别、年龄、乙肝、肝硬化、肿瘤包膜等临床病理特征间无明显相关(P>0.05);而与肿瘤大小、血管侵犯情况及临床分期等因素间显著相关(P<0.05);多因素Logistic回归分析发现,TNM分期是TET1低表达的独立危险因素(P=0.000,OR=12.508,95%CI:3.484-44.914)。结论:TET1在HCC中表达下调,并可能对HCC的发生发展具有重要作用。

Objective:To investigate the expression and clinical significance of ten-eleven translocation 1(TET1)in patients with hepatocellular carcinoma(HCC),and to explore the relationship between clinicopathologic characteristics and TET1 protein expression.Methods:The mRNA and protein expression of TET1 in tumor and matched adjacent non-cancerous liver tissues from 126 patients with HCC was evaluated by real-time reverse transcription PCR and immunohistochemistr using tissue microarray technology.The relationship between the expression of TET1 and the clinicopathological features in patients with HCC was also analyzed.Results:The expression of TET1 mRNA was markedly reduced in HCC tissues(0.07[0.01,0.14])compared with matched adjacent non-cancerous liver tissues(1[1,1])(P<0.001).Negative and weak staining of TET1 was observed in 77.0%(97/126)of the HCC tissue samples,while strong staining was found in72.2%(91/126)of the adjacent non-cancerous liver tissue samples.TET1 was significantly decreased in HCC tissues(P<0.01).The expression of TET1 was not obviously related to sex,age,HBV status,liver cirrhosis,Edmondson-Steiner grade,or capsular invasion(P>0.05).However,the expression of TET1 was significantly correlated with tumor size,vascular inva-sion,and TNM stage(P<0.05).Multiple logistic regression analysis showed that the TNM stage(P=0.000,OR=12.508,95% CI:3.484-44.914)was the independent risk factor for the decreased expression of TET1.Conclusion:TET1 is down-regulated in HCC.And it also suggests that the decreased expression of TET1 may have an important role in the development of hepatocellular carcinoma.