慢性阻塞性肺疾病患者外周血Th17和Treg细胞的表达及意义

Expression and Clinical Significance of Th17 Cells and Regulate T Cells in Peripheral Blood of Chronic Obstructive Pulmonary Disease

目的:探究慢性阻塞性肺疾病(COPD)患者外周血Th17和调节性T细胞(Treg)的表达及临床意义。方法:选择2015年6月-12月在本院住院治疗的慢性阻塞性肺疾病急性加重期患者(AECOPD)25例、慢性阻塞性肺疾病稳定期(SCOPD)患者21例、健康吸烟者17例和健康不吸烟者16例作为研究对象。采用流式细胞术检测外周血中Th17细胞、Treg细胞的比例,Bio-plex悬液芯片技术检测血清中相关细胞因子的水平。结果:AECOPD、SCOPD期组患者外周血中Th17细胞比例和细胞因子IL-1β、IL-6、IL-17A水平明显高于对照组(P<0.01),AECOPD组中Treg细胞比例和IL-10水平低于SCOPD组和对照组(P<0.01),AECOPD组、稳定期组中Th17比例与Treg比例均呈负相关(r=-0.770、-0.788,均P<0.01),SCOPD组Th17/Treg比值与FEV1%、FEV1/FVC呈负相关(r分别为-0.815、-0.763,均P<0.05)。结论:COPD患者外周血中Th17/Treg细胞表达失衡,存在免疫内环境的紊乱,可能参与了COPD的发病和急性加重过程。

Objective:To investigate the expression and clinical significance of Th17 cells and regulate T cells(Treg)in peripheral blood of chronic obstructive pulmonary disease(COPD).Methods:A total of 25 patients of acute exacerbation chronic obstructive pulmonary disease(AECOPD),21 patients of stable chronic obstructive pulmonary disease(SCOPD),17 health smokers and 16 health non-smokers were recruited in this study.Th17 cells and Treg cells in peripheral blood were detected by flow metometry.The cytokines levels in serum were detected by The Bio-Plex Suspension Array System.Results:The percentage of Th17 cells,L-1β,IL-6,and IL-17 Alevels were significant higher in AECOPD and SCOPD groups(P<0.01).However,the percentage of Treg cells and IL-10 levels were lower in AECOPD group(P<0.01).There was a negative correlation between the percentage of Th17 and Treg cells in AECOPD and SCOPD(r=-0.770,-0.788,P<0.01),the ratio of Th17/Treg was inversely correlated with FEV1%and FEV1/FVC(r=-0.815,-0.763,P<0.05)in SCOPD.Conclusion:Th17/Treg cells imbalance might be involved in the pathogenesis and acute exacerbation of COPD.