miR-146a和LIN52在甲状腺癌中表达水平及与化疗敏感性和预后的关系

Expression of miR-146a and LIN52 in thyroid carcinoma and its relationship with chemotherapeutic sensitivity and prognosis of patients

目的探讨miR-146a和LIN52在甲状腺癌中表达水平及其与化疗敏感性和预后的关系。方法选取确诊的甲状腺癌患者98例,收集患者癌组织与癌旁组织标本,采用逆转录实时荧光定量法和免疫组化法测定miR-146a、LIN52的表达水平,分析其与临床病理特征、化疗敏感性及预后的关系。结果癌组织中miR-146a mRNA表达量低于癌旁组织,LIN52 mRNA表达量高于癌旁组织(t=36.182、44.023,P<0.01);癌组织miR-146a阳性率低于癌旁组织,LIN52阳性率高于癌旁组织(χ~2=111.942、100.178,P<0.01);miR-146a、LIN52 mRNA表达与年龄相关性不明显(χ~2=0.248、0.284,P>0.05),与病理学分级、TMN分期、浸润深度、淋巴血管间隙浸润、淋巴结转移及化疗敏感性相关性明显,且病理学分期越高、TMN分期越高、浸润深度越深、淋巴血管间隙浸润、有淋巴结转移、化疗敏感性低患者miR-146a阳性表达率较低,LIN52阳性表达率较高(χ~2=19.934、10.292、25.514、7.728、8.738、27.774、20.327、10.458、32.932、6.756、13.162、18.506,P<0.05);miR-146a阳组3年生存率及生存期均明显高于miR-146a阴组,LIN52阳组3年生存率及生存期均明显低于LIN52阴组(χ~2=64.542、27.993、29.142、22.982,P<0.01)。结论 miR-146a在甲状腺癌组织的表达量低于癌旁组织,而LIN52表达量高于癌旁组织;miR-146a与化疗敏感性正相关,而LIN52与化疗敏感性负相关,miR-146a表达阳性、LIN52表达阴性的患者能获得较好的预后。

Objective To investigate the expression of miR-146 a and LIN52 in thyroid carcinoma and its relationship with chemotherapeutic sensitivity and prognosis of patients.Methods A total of 98 patients with thyroid carcinoma who were definitely diagnosed in our hospital were enrolled in the study.The specimens of cancer tissue and paracancerous tissue were collected.The expression levels of miR-146 a and LIN52 were detected by reverse transcriptase by real-time fluorescence quantitative PCR and immunohistochemical method.The relationship between the expression levels of miR-146 a,LIN52 and chemosensitivity,clinical features,patient’s prognosis was analyzed.Results The expression levels of miR-146 a mRNA in cancer tissues were significantly lower than those in cancer adjacent tissues,however,the expression levels of LIN52 mRNA in cancer tissues were significantly higher than those in cancer adjacent tissues(P<0.01).The positive rate of miR-146 a in cancer tissues was significantly lower than that in cancer adjacent tissues,but the positive rate of LIN52 was significantly higher than that in cancer adjacent tissues(P<0.01).The expression levels of MiR-146 a and LIN52 mRNA were not obviously correlated with age(P>0.05),however,which were closely correlated with pathological grade,TMN stage,invasion depth,lymph vascular space invasion,lymph node metastasis and chemotherapy sensitivity,moreover,the higher pathological staging,TMN staging,infiltration depth,lymph vascular space invasion,lymph node metastasis and lower sensitivity to chemotherapy,the lower the miR-146 a positive expression rates and the higher the LIN52 positive expression rates(P<0.05).Moreover the 3-year survival rate and survival duration in miR-146 a positive expression group were significantly higher than those in miR-146 a negative expression group,and the 3-year survival rate and survival duration in LIN52 positive expression group were significantly lower than those in LIN52 negative expression group(P<0.01).Conclusion The expression levels of miR-146 a in thyroid carcinoma tissues are lower than those in paracancerous tissues,however,the expression levels of LIN52 are higher than those in paracancerous tissues.MiR-146 a expression is positively correlated with chemosensitivity,but LIN52 expression is negatively correlated with chemosensitivity.The thyroid cancer patients with positive expression of miR-146 a or negative expression of LIN52 can achieve better prognosis.