miRNA-136在肝癌组织中的表达及临床意义

Expression and clinical significance of miRNA-136 in hepatocellular carcinoma

目的探讨miRNA-136在肝癌组织中的表达及临床意义。方法选取2013年1月至2014年12月收治的62例经手术病理证实的原发性肝癌患者为研究对象。采用qRT-PCR法检测患者肝癌组织及癌旁正常组织中miRNA-136的表达水平,并分析其与肝癌患者临床病理特征及预后的相关性。结果 miRNA-136在肝癌组织中的表达明显高于癌旁正常组织(3.57±1.14 vs 1.18±0.45),差异有统计学意义(P<0.05);肝癌组织中miRNA-136的表达与AJCC分期、淋巴结转移存在相关性(P<0.05),而与肝癌患者性别、年龄、肿瘤大小、肝硬化、乙肝及分化程度无明显关系(P>0.05);miRNA-136高表达组肝癌患者3年生存率(21.05%)明显低于低表达组(55.56%),2组比较差异有统计学意义(P<0.05);Cox分析显示,miRNA-136高表达是影响肝癌患者预后的独立危险因素。结论 miRNA-136在肝癌组织中呈高表达,其可能参与肝癌的发生发展,或将成为肝癌临床诊疗及预后评价的新指标。

Objective To investigate the expression and clinical significance of miRNA-136 in hepatocellular carcinoma.Methods Sixty-two patients with pathologically confirmed primary hepatocellular carcinoma who were admitted and treated in our hospital from January 2013 to December 2014 were enrolled in the study.The expression levels of miRNA-136 were detected by qRT-PCR in hepatocellular carcinoma tissues and adjacent normal tissues to analyze its correlation with clinicopathological features and prognosis of patients.Results The expression levels of miRNA-136 in hepatocellular carcinoma tissues were significantly higher than those in adjacent normal tissues(3.57±1.14 vs 1.18±0.45, P<0.05).There was a correlation between miRNA-136 expression and AJCC staging and lymph node metastasis in HCC tissues(P<0.05).However,there was no significant correlation between between miRNA-136 expression and patient’s gender and age,tumor size,liver cirrhosis,hepatitis B and the degree of differentiation of liver cancer(P>0.05).The 3-year survival rate(21.05%) in patients with high expression of miRNA-136(21.05%) was significantly lower than that(55.56%) in patients with low expression of miRNA-136(P<0.05).Cox analysis showed that miRNA-136 overexpression was an independent risk factor influencing prognosis of patients with liver cancer.Conclusion The miRNA-136 is highly expressed in hepatocellular carcinoma,which may be involved in the pathogenesis and development of liver cancer, and it may become an new index to evaluate the clinical diagnosis and prognosis of liver cancer.