原发性肝癌患者疼痛与血清HIF-1及VEGF水平的相关性分析

Correlation Analysis on the Relationship between Pain and Serum HIF-1 and VEGF Levels in Patients with Primary Liver Cancer

目的:探讨分析原发性肝癌患者疼痛与血清缺氧诱导因子-1(HIF-1)及血管内皮生长因子(VEGF)水平的相关性。方法:选取我院2016年1月至2017年11月我院收治的肝癌患者94例,根据视觉模拟评分法(VAS)评分,将患者分为无疼痛组30例,一般疼痛组39例和严重疼痛组25例,所有患者取肝癌组织制作病理标本,采用RT-PCR法检测肝癌组织内HIF-1 mRNA及VEGF mRNA的表达量。ELISA法检测血清HIF-1及VEGF的水平。结果:三组VAS评分具有明显差异(P<0.05)。严重疼痛组HIF-1 mRNA及VEGF mRNA相对表达量、血清HIF-1及VEGF水平均明显高于无疼痛组和一般疼痛组,一般疼痛组HIF-1 mRNA及VEGF mRNA相对表达量、血清HIF-1及VEGF水平均明显高于无疼痛组,差异具有统计学意义(P<0.05)。VAS评分与HIF-1和VEGF呈正相关(r>0,P<0.05)。结论:HIF-1及VEGF在肝癌患者中的表达程度与患者疼痛程度具有一定的相关性,为临床缓解肝癌患者疼痛提供了新思路,具有重要的临床价值。

Objective: To investigate the relationship between pain and serum levels of hypoxia-inducible factor-1 (HIF-1) and vascular endothelial growth factor (VEGF) in patients with primary liver cancer.Methods: From January 2016 to November 2017,94 patients with hepatocellular carcinoma were divided into three groups according to visual analogue scale (VAS). Thirty patients were divided into pain-free group,39 patients in general pain group and 25 patients in severe pain group. The expression level of HIF-1 and VEGF mRNA,serum levels of HIF-1 and VEGF were measured by ELISA. Results: There was a significant difference in VAS between the three groups (P <0.05). The relative expression of HIF-1 mRNA and VEGF mRNA,serum HIF-1 and VEGF levels in severe pain group were significantly higher than those in painless group and general pain group. The relative expression of HIF-1 mRNA and VEGF mRNA,serum HIF-1 and VEGF levels in general pain group were significantly higher than those in painless group (P <0.05). VAS score was positively correlated with HIF-1 and VEGF (r > 0,P < 0. 05). Conclusion: The expression of HIF-1 and VEGF in patients with hepatocellular carcinoma has a certain correlation with the degree of pain,which provides a new idea for clinical relief of pain in patients with hepatocellular carcinoma and has important clinical value.