二氧化碳麻醉技术在罗非鱼无水保活运输中的应用研究

The application research of CO_2 in alive-keeping and transportation without water of tilapia

采用二氧化碳(CO2)对罗非鱼进行麻醉,以麻醉时间、复苏时间为指标,探讨CO2浓度、水温、浸浴时间对罗非鱼麻醉效果的影响。结果显示,水温25℃、浓度0.41~1.13 mg/L时,随着浓度的增加,麻醉时间缩短,复苏时间延长;浓度0.58 mg/L时,水温由10℃升高到30℃时,麻醉时间呈先上升后下降的趋势,复苏时间呈下降趋势。罗非鱼血浆生化指标分析表明,麻醉中罗非鱼的乳酸脱氢酶、谷草转氨酶、谷丙转氨酶活性分别与麻醉前、麻醉后差异显著(P【0.05);麻醉中罗非鱼的肌酐、尿素氮、乳酸含量显著高于麻醉前和复苏后;与麻醉中罗非鱼的血浆生化指标相比,复苏后的罗非鱼血浆生化指标均下降。研究表明,CO2对罗非鱼有麻醉作用,CO2麻醉可辅助罗非鱼无水保活运输。

In order to investigate the anesthetic effect of carbon dioxide concentrations,water temperature and bath time on tilapia,tilapias were anaesthetized by carbon dioxide in this study,with anesthetic time and recovery time as indicators. The results showed that at the water temperature of 25 ℃,the anesthetic time decreased and recovery time rose when the concentrations of CO2 rose from 0. 41 to 1. 13 mg /L. At the concentration of 0. 58 mg /L,the anesthetic time showed a downward trend after the first rising and recovery time decreased when the water temperature rose from 10 ℃ to 30 ℃. The results of the analysis of blood plasma indicators of tilapias showed that the activity of lactate dehydrogenises, aspartate aminotransferase and aminotransferase of anaesthetized tilapias were significantly different from those of pro-anaesthetized tilapia and post-anaesthetized tilapias respectively( P < 0. 05). The concentrations of urea nitrogen,creatinine,and lactate of anaesthetized tilapias were significantly higher than those of pro-anaesthetized and post-anaesthetized tilapias.Compared with plasma biochemical indicators of anaesthetized tilapias,the plasma biochemical indicators of post-anaesthetized tilapias decreased. This study showed that carbon dioxide had a good anesthetic effect on tilapias,and it can be used to assist alive-keeping and transportation without water of tilapias.