石竹愈伤组织诱导及植株再生

The Induction of Callus and Plantlet Regeneration in Dianthus chinensis

通过诱导愈伤组织再分化和直接诱导不定芽这两种途径获得了石竹的再生植株.比较了不同植物生长调节剂配比在离体培养中的效应,认为6 BA是诱导长期培养的愈伤组织再分化最有效的外源激素,适宜的质量浓度范围在0 5～2 0mg/L之间,当6 BA为1 0mg/L,NAA为0 3mg/L时,愈伤组织不定芽分化频率最高,小苗生长状况也最好;将6 BA与NAA、IBA、Ad等生长调节剂混合使用可提高诱导效果.增殖继代培养基中6 BA的质量浓度在0 1～0 3mg/L之间为宜,浓度过大,虽然芽增殖数量增多,但苗的畸形化和愈伤化现象严重.

The regenerated plantlets in Dianthus chinensis were obtained by two methods: through the redifferentiation of the callus and the adventitious buds induced directly from the explant. The effects of plant regulator compositions on the differentiation of callus and the induction of adventitious buds in vitro culture were studied. The propagation of the regenerated plants and the induction of the roots were also discussed in this report. 