全磷和缺磷条件下甘蔗叶悬浮细胞主要生长指标的比较

Comparison of the Major Growth Indexes of Sugarcane Leave Suspension Cell Under Phosphate Sufficiency and Deficiency

研究了以甘蔗基因型US66 56 9的心叶为材料,对甘蔗愈伤组织进行诱导和建立甘蔗叶片悬浮细胞系,并对悬浮细胞进行全磷和缺磷处理,然后对二者的生长曲线、细胞含水率及培养基pH等变化进行了比较.结果表明:与全磷条件相比,缺磷胁迫抑制细胞的生长,导致细胞含水率较高,使培养基的pH值在生长过程中基本保持酸性状态.

The genotype US66-56-9 of sugarcane leave was used as the material and to induce and to establish suspension cell culture system. Under the condition of phosphate sufficiency and deficiency, the comparison was made in terms of the changes of growth curves, cell water content rates and medium pH values of suspension cell, respectively. The results were followed: compared with the condition of phosphate sufficiency, phosphate deficiency inhibited cell growth, kept cell water content rate higher, maintained pH value of medium with acidic throughout cell growth.