J亚群禽白血病病毒gp37基因的克隆和序列分析

Clone and Sequencing of gp37 of Subgroup J Avian Leukosis Viruses in China

近两年我们从国内不同省份先后分离和鉴定了 8株J亚群禽白血病病毒 (ALV_J) ,并对其中的 5株进行了gp3 7基因的克隆和序列分析。结果表明 ,我们分离的 5株ALV_J之间的同源性为 94.6%～ 99.0 % ;与国内最早分离的SD990 1、SD990 2和YZ990 1等毒株之间的同源性分别为 95 .3 %～ 98.5 %、95 .6%～ 99.0 %和 94.9%～ 98.6% ;与国际最先报道的原型株HPRS_1 0 3之间的同源性为 93 .4%～ 95 .1 % ;与其它国外毒株的同源性为 92 .7%～ 96.8%。这表明我国ALV_J的gp3 7基因正在不断发生变异 ,但相对于gp85基因的变异性要小。

Envelope glycoprotein(gp37)of subgroup J avian leukosis virus(ALV-J)were amplified and anlysised in 5strains SD0001,SD0002,SD0101,HN0001and NX0101 from 8 strains which isolated from different provinces in China.Results showed that gp37 of our strains had 94.6%～99.0% mutual homology in the amino level,95.3%～98.5%?95.6%～99.0% and 94.9%～98.6% comparing to ALV-J strain SD9901,SD9902 and YZ9901 which was isolated in China firstly,93.4%～95.1% comparing with prototype strain HPRS-103 and 92.7% ～96.8% with other strains abroad.It was concluded that changes of gp37 was less than gp85 from strains in China,but it was still taking place.