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高糖刺激人脐静脉内皮细胞丝裂素活化蛋白激酶P38的表达 被引量：4

High glucose level stimulates P38 mitogen - actived protein kinase expression in human umibilical vein endothelial cell
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摘要 目的 探讨丝裂素活化蛋白激酶P38(P38MAPK)在糖尿病血管并发症中的作用。方法 采用体外培养和Western-blot等方法,以不同浓度D-葡萄糖及不同时间刺激人脐静脉内皮细胞系,分别检测人脐静脉内皮细胞系P38MAPK的蛋白表达。结果 随D-葡萄糖浓度增加,P38MAPK磷酸化蛋白表达量增加;延长刺激时间,P38MAPK磷酸化蛋白表达量亦随之增加。结论高糖引发的P38MAPK表达异常,可能在糖尿病血管并发症的发生、发展中起关键作用。 Objective To study the effect of P38 mitogen-actived protein kinase(P38MAPK)on diabetic vascular complication. Methods Human umibilical vein endothelial cell line cultured in RPMI1640 medium was stimulated by D-glucose with different concentration and time respectively. Expression of P38MAPK in human umibilical vein endothelial cell line was detected. Results Expression of P38MAPK increased as the concentration and time of D-glucose got larger. Conclusion P38MAPK over expression induced by hyperglycemia may act as a key effect in the development of diabetic vascular complication.
作者 李艳波 邓华聪 郑丹 韩君勇 李呼伦
机构地区 重庆医科大学附属第一医院内分泌科 重庆医科大学附属第一医院心内科 哈尔滨医科大学微生物学教研室
出处 《哈尔滨医科大学学报》 CAS 2002年第6期433-435,共3页 Journal of Harbin Medical University
基金 国家自然科学基金资助项目(30070356)
关键词 高糖 丝裂素活化蛋白激酶P38 糖尿病 血管并发症 人脐静脉内皮细胞 high glucose level P38 mitogen-actived protein kinase diabetes mellitus vascular complication human umibilical vein endothelial cell
分类号 R587.1 [医药卫生—内分泌] R543 [医药卫生—心血管疾病]
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参考文献7

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同被引文献45

  • 1刘小鹏,罗春英,曹仁贤,刘江华,文芳,李云,文格波.高糖作用下脐静脉内皮细胞中血管内皮生长因子基因表达与蛋白激酶C通路的关系[J].中国动脉硬化杂志,2006,14(1):17-20. 被引量：8
  • 2Jameel N M, Thirunavukkarasu C, Wu T, et al. p38-MAPK-and caspase-3-mediated superoxide-induced apoptosis of rat hepatic stellate cells : reversal by retinoic acid [ J ]. J Cell Physiol, 2009, 218 ( 1 ) : 157 - 166.
  • 3Alikhani M, Maclellan C M, Raptis M, et al. Advanced glycation end products induce apoptosis in fibroblasts through activation of ROS, MAP kinases, and the FOXOI transcription factor[ J ]. Am J Physiol Cell Phvsiol, 2007, 292(2) : C850- C856.
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  • 6Wang X, Goh C H, Li B. p38 mitogen-activated protein kinase regulates osteoblast differentiation through osterix [ J ]. Endocrinology, 2007, 148(4) : 1629 -1637.
  • 7Wei L, Sun X J, Wang Z, et al. CD95-induced osteoarthritic chondrocyte apoptosis and necrosis: dependency on p38 mitogen-activated protein kinase[J]. Arthritis Res Ther, 2006, 8(2) : 37 -41.
  • 8Tanaka Y, Nakayamada S, Fujimoto H, et al. H-Ras/mitogen-aetivated protein kinase pathway inhibits integrin-mediated adhesion and in duces apoptosis in osteoblasts [ J ]. J Biol Chem, 2002, 277 (24) : 21446 - 21452.
  • 9Alikhani M,Alikhani Z, Boyd C, et al. Advanced glycation end products stimulate osteoblast apoptosis via the MAP kinase and cytosolic apoptotic pathways[J]. Bone, 2007, 40(2) : 345 -353.
  • 10Qi J s, Li Y N, Zhang B S, et al. Peroxynitrite mediates high glucose-induced osteoblast apoptosis[J]. J Endocrinol Invest, 2008, 31 (4) : 314 -320.

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哈尔滨医科大学学报
2002年 第6期

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