乙型肝炎病毒c基因启动子变异与血清HBeAg及HBV DNA的关系

Relationship between HBV c gene promoter gene mutation and serum HBeAg and HBV DNA contents

目的 :研究乙型肝炎病毒 (HBV ) c基因启动子 (BCP)变异与 e抗原及 HBV DNA含量的关系。方法 :采用错配引物聚合酶链反应 (PCR) -限制性片段长度多态性 (RFL P)分析及荧光定量 PCR(FQ- PCR)对 10 5例血清 HBV DNA阳性 HBV感染者进行 HBV BCP基因变异及 HBV DNA定量测定。结果 :BCP变异组 HBe Ag阴性率为 84 .4 % (5 4 / 6 4 ) ,非 BCP变异组为2 6 .8% (11/ 4 1)。 BCP变异组 HBV DNA含量在 HBe Ag+ 病例 (10 8.79± 0 .85vs 10 7.4 8± 0 .39copy/ ml,P <0 .0 1)及 HBe Ag- 病例(10 8.0 5± 1 .1 6 vs10 6 .55± 0 .91 copy/ ml,P<0 .0 1)中均较非 BCP变异组高。结论 :HBV BCP变异在下调前 c/ c基因表达的同时可能促进 HBV复制 ,对 HBs Ag+ / HBe Ag- 患者需要进一步检测 HBV DNA,以免由于基因变异导致将 HBe

Objective: To investigate the relationship between HBV basic core promoter(BCP) mutation and serum HBeAg and HBV DNA contents in HBV infected patients.Methods: Mismatched primer polymerase chain reaction restriction fragment length polymorphism(PCR RFLP) analysis and fluorescent quantitative PCR(FQ PCR) were applied in the analysis of HBV BCP gene mutation and HBV DNA contents in 105 HBV DNA positive patients.Results: The negative rate of HBeAg were 84.4%(54/64) and 26.8%(11/41) in patients with BCP mutation and non BCP mutation.The quantity of HBV DNA was significantly higher in HBV BCP mutant group than non mutant group either in patients with HBeAg + (10 8.79±0.85 vs 10 7.48±0.39 copy/ml, P <0.01) or HBeAg - (10 8.05±1.16 vs 10 6.55±0.91 copy/ml, P <0.01).Conclusion: The reduction of pre c/c gene expression in patients with BCP mutation is accompanied by an increase in HBV replication.In HBV infected patients with HBsAg +/HBeAg -,which are often conceived to have virus immune clearance or rest of virus replication,HBV DNA content should be tested to avoid missing the occasion of antiviral therapy.