脂多糖诱导滑膜细胞产生一氧化氮及木黄酮对其的抑制作用

Lipopolysaccharide induced NO production in synoviocytes and inhibitory effect of genistein on it

目的 :研究脂多糖 (L PS)诱导滑膜细胞产生一氧化氮 (NO)及酪氨酸激酶 (PTK)抑制剂木黄酮对其的抑制作用。 方法 :L PS或 L PS+木黄酮体外刺激滑膜细胞 ,用 Griess方法检测上清中 NO的含量。结果 :L PS能诱导滑膜细胞产生 NO,此作用具有时间 (0～ 72 h)和剂量依赖性 (0 .0 1～ 10 0μg/ml) ;木黄酮 (6 .2 5～ 5 0μmol/L )剂量依赖性地抑制 L PS诱导滑膜细胞产生 NO,5 0 μmol/L 木黄酮抑制率达到 5 0 .2 2 %。 结论 :木黄酮能剂量依赖性地抑制 L PS诱导滑膜细胞产生 NO。

Objective: To study the effect of lipopolysaccharides(LPS) on nitric oxide(NO) production in synoviocytes and inhibition of genistein on it.Methods: LPS or LPS+genistein stimulated synoviocytes in vitro .NO in culture medium was determined by Griess method.Results: LPS dose and time dependently induced NO production in synoviocytes; Genistein (6.25 50 μmol/L) dose dependently inhibited the production of NO induced by lipopolysaccharide.Conclusion:The effect of LPS on NO production can be dose dependently inhibited by genistein,this may be mediated by tyrosine kinase(PTK).